Heat-treated limulus amebocyte lysates
AssigneeCharles River Laboratories, Inc.
InventorMasakazu Tsuchiya
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Abstract
The application provides heat-treated Limulus amebocyte lysates useful for detecting β-glucans.
Claims48
A method of preparing a Limulus amebocyte lysate with increased specificity for (1→3)-β-D-glucan, the method comprising the step of heating the Limulus amebocyte lysate to a temperature above 40° C. to reduce sensitivity to an endotoxin, with the proviso that the lysate is not heated to 56° C. for 30 minutes.
A method of preparing a Limulus amebocyte lysate having reduced sensitivity to an endotoxin, the method comprising the step of heating the Limulus amebocyte lysate to reduce Factor C activity in the lysate while retaining sensitivity to (1→3)-β-D-glucan.
Dependencies are inferred from the claim text.