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US 10,473,645 B2Granted
Methods for identifying a receptor for a ligand and uses thereof
AssigneeUniversite De Geneve
InventorsIvan Rodriguez, Alan Jacques Henri Cyrus Carleton, Daniel Rossier
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Abstract
The present invention relates to a novel method for identifying pairs of receptors/ligands, transgenic animals useful for carrying out said method, and the use of ligands and/or modulators of the interaction between a ligand and its receptor in the food industry, fragrance industry, and health industry, for instance.
Claims12
A method of identifying at least one olfactory receptor responding to at least one ligand comprising the steps of:
- a) providing a biological sample comprising cells expressing at least one olfactory receptor, wherein said biological sample (i) has been exposed to at least one test compound or (ii) was obtained from an animal that has been exposed to at least one test compound;
- b) measuring the level of mRNAs encoding at least one olfactory receptor in said biological sample, and
- c) comparing the level of mRNAs determined in step b) to the level of mRNAs determined in the same conditions with a negative control where the biological sample or animal has not been exposed to said at least one test compound;
- wherein a difference between the level of mRNAs determined in step b) and the level of mRNAs determined in the same conditions with a negative control indicates that said at least one olfactory receptor responds to said at least one test compound which constitutes a ligand for said at least one olfactory receptor and is able to bind and modulate the activity of said at least one olfactory receptor.
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Dependencies are inferred from the claim text.