Nozzle and method for flow cytometry
AssigneeMasterrind GmbH
InventorDetlef Rath
Life of this patent
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Abstract
The invention relates to a nozzle for flow cytometry, the housing of which is tapering towards an outlet and in which a feed tube is arranged for a core flow liquid, the outlet opening of which is arranged at a distance from the outlet of the housing. The outlet of the housing forms the outlet of the nozzle. The housing of the nozzle extends from its outlet, which is arranged at its first end to its opposite second end, and has an inlet for a sheath flow liquid connected with the internal volume. The nozzle is characterized in that in the housing a leading element that promotes the alignment of particles extends from both sides of the feed tube.
Claims14
A method for producing a liquid flow having a particle-containing core flow surrounded by a sheath flow by introducing a particle-containing core flow liquid into an inlet opening for core flow liquid and introducing a sheath flow liquid into an inlet opening for sheath flow liquid of a nozzle, wherein the sheath flow is generated by a leading element that extends along a longitudinal axis to a larger degree along a first dimension perpendicular to the longitudinal axis up to a distance away from the inner wall of a housing than it extends in a second dimension that is perpendicular to the longitudinal axis and perpendicular to the first dimension, wherein the leading element extends from a first end, which lies in the plane of the first end of a feed tube or is offset by a distance from the first end of the feed tube, up to its second end, which is arranged at a distance from the second end of the nozzle, wherein the leading element in the nozzle extends on both sides of the feed tube for aligning the particles which are contained in the core flow, wherein the extension of the leading element in its second dimension causes different flow velocities of the sheath flow liquid, which different flow velocities lead to an alignment of the particles, and wherein the particles have the alignment also after outflow from the outlet of the nozzle.
A method for producing a liquid flow having a particle-containing core flow surrounded by a sheath flow by introducing a particle-containing core flow liquid into an inlet opening for core flow liquid and introducing a sheath flow liquid into an inlet opening for sheath flow liquid of a nozzle, wherein the sheath flow is generated by a leading element that extends along a longitudinal axis to a larger degree along a first dimension perpendicular to the longitudinal axis up to a distance away from the inner wall of a housing than it extends in a second dimension that is perpendicular to the longitudinal axis and perpendicular to the first dimension, wherein the leading element extends from a first end, which lies in the plane of the first end of a feed tube or is offset by a distance from the first end of the feed tube, up to its second end, which is arranged at a distance from the second end of the nozzle, wherein the leading element in the nozzle extends on both sides of the feed tube for aligning the particles which are contained in the core flow, such that particles with a stretched cross section are brought into a common alignment and wherein the particles have the alignment also after outflow from the outlet of the nozzle.
Dependencies are inferred from the claim text.