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US 10,473,567 B2Granted
Cell collecting device
AssigneeCytogen Corporation
InventorsByung Hee Jeon, Jong Kil Lee
Life of this patent
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Abstract
A cell collecting device is configured to collect target cells from a fluid sample such as blood or physiological fluid. The cell collecting device includes a conduit through which a fluid sample containing target cells passes. The cell collecting device further includes a screen filter installed inside the conduit and configured to guide the target cells toward a specified region. The screen filter includes a plurality of filtering holes for filtering the target cells.
Claims10
A method of collecting cells from a fluid sample, the method comprising:
- providing a cell collection device comprising a first body, a second body and a screen device, the first body comprising a first sidewall defining a first conduit, the first sidewall having a first bottom surface, the second body comprising a second sidewall defining a second conduit, the second sidewall having a second top surface and a second bottom surface, the screen device comprising a screen portion with a plurality of pores, wherein the first body is placed over the second body such that the first bottom surface of the first sidewall faces the second top surface of the second sidewall and further such that the first and second conduits are aligned to provide a unitary conduit, wherein the screen device is engaged with at least one of the first and second body such that at least part of the screen portion is placed inside the unitary conduit;
- providing a fluid composition comprising target cells that are large enough to not pass through the plurality of pores;
- pouring the fluid composition from over the cell collection device into the unitary conduit such that the fluid composition flows downward, wherein at least part of the target cells do not pass through the plurality of pores and stays over the screen portion while at least part of the rest of the fluid composition passes downward through the plurality of pores of the screen portion;
- and
- subsequently, collecting at least part of the target cells that has not passed through the plurality of pores,
- wherein the second bottom surface is generally perpendicular to a downward flow direction defined by the unitary conduit, wherein the screen portion is slanted relative to the second bottom surface, wherein the slanted screen portion provides the lowest region on the screen portion, wherein at least part of the target cells slide over the screen portion toward the lowest region,
- wherein the lowest region on the screen portion in adjacent to an inner surface of the first sidewall, wherein the cell collection device comprises a through-hole next to the lowest region on the screen portion for discharging at least part of the target cells from unitary conduit therethrough, wherein at least part of the target cells are collected from outside the through-hole.
4 more elements
Dependencies are inferred from the claim text.