US 10,472,678 B2 · Reagent for detecting cross contamination of PDX-model related with human and mouse, the kit comprising the same, and the method for the cross contamination detection

Abstract

The present application relates to a detection kit for genotypes capable of confirming cross contamination that may occur in a banking process of a patient-derived xenograft model or cell-derived xenograft model and a method for determining cross contamination using the same. According to the present invention, it is possible to determine all of cross contamination of mouse related genes, have high detection sensitivity and specificity to be close to 100%, rapidly examine the contamination, and be very useful in predicting mouse contamination. Therefore, according to the present invention, cross contamination of genes related with the human and the mouse is predicted in advance to be applied to evaluation of anticancer drug efficacy using a patient-derived xenograft model or cell-derived xenograft model and contribute to cell banks using the patient-derived xenograft model or cell-derived xenograft model, and as a result, the present invention is very useful in a medical industry.

Claims6

Dependent claims

A detection kit that determines patient-derived xenograft cells, comprising:

oligonucleotide primers consisting of base sequences of SEQ ID NOS:
9 and 10 and oligonucleotide probes consisting of base sequences of SEQ ID NOS:
9 and 10 and an attached label, wherein the primers and the probes determines a ratio of genes included in xenograft cells of a human and a mouse.

Dependencies are inferred from the claim text.

Facts

Application

15/582,773Filed 1 May 2017

Priority

KR 10-2016-00544583 May 2016

Published

US 2017/0321275 A19 Nov 2017

Granted

12 Nov 2019

Term ends

Not estimatedThe source records don’t support an estimate.

Classification4 codes

C12Q 1/6881
Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes: for tissue or cell typing, e.g. human leukocyte antigen [HLA]…
C12Q 1/6888
Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes: for detection or identification of organisms
C12Q 2600/158
Oligonucleotides characterized by their use: expression markers
C12Q
Measuring or testing processes involving enzymes, nucleic acids or microorganisms

Reagent for detecting cross contamination of PDX-model related with human and mouse, the kit comprising the same, and the method for the cross contamination detection

Life of this patent

Reagent for detecting cross contamination of PDX-model related with human and mouse, the kit comprising the same, and the method for the cross contamination detection

Life of this patent

Abstract

Claims6