System and method for targeted depletion of nucleic acids
AssigneeRoche Sequencing Solutions, Inc.
InventorsVenera Bouriakov, Daniel Burgess
Life of this patent
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Abstract
The present disclosure provides a system and method for depleting target nucleic acids from a nucleic acid sample. In one aspect, a kit according to the present disclosure includes a plurality of DNA probes. Each of the DNA probes is hybridizable to form a heteroduplex with at least one of a plurality of target RNA transcripts in a nucleic acid sample. The number of unique target RNA transcripts hybridized by the plurality of DNA probes is at least three. The kit further includes an enzyme having RNA-DNA hybrid ribonucleotidohydrolase activity, where degrades at least the RNA portion of the heteroduplex.
Claims11
A method for depleting target nucleic acids from a nucleic acid sample, the method comprising:
- hybridizing a plurality of deoxyribonucleic acid (DNA) probes with a plurality of target ribonucleic acid (RNA) transcripts in a nucleic acid sample, each of the DNA probes forming a heteroduplex with at least one of the plurality of target RNA transcripts;
- and
- treating the heteroduplex with an enzyme having RNA-DNA hybrid ribonucleotidohydrolase activity, thereby degrading at least the RNA portion of the heteroduplex,
- wherein the number of unique target RNA transcripts hybridized by the plurality of DNA probes is at least 3, and
- wherein the DNA probes are hybridizable along the length of each of the target RNA transcripts with a regular spacing, wherein for each pair of adjacently hybridizable DNA probes, the 3′ end of a first one of the pair of DNA probes is spaced apart from the 5′ end of a second one of the pair of DNA probes by an interval of nucleotides relative to the target RNA transcript.
Dependencies are inferred from the claim text.