Measuring method and composition using kinase
AssigneeAsahi Kasei Pharma Corporation
InventorsShigeru Ueda, Shinichi Sakasegawa
Life of this patent
Estimating the term…
Loading patent…
AssigneeAsahi Kasei Pharma Corporation
InventorsShigeru Ueda, Shinichi Sakasegawa
Estimating the term…
The present invention provides a measuring method for at least one of a kinase forward reaction substrate, a phosphorylated product thereof, and a precursor thereof, and includes a step of conducting an enzymatic cycling reaction by bringing at least a kinase, a first nucleotide coenzyme of the kinase, and a second nucleotide coenzyme having a different nucleoside moiety from the first nucleotide coenzyme into contact with a sample; a step of detecting a signal corresponding to a change of at least one of the first nucleotide coenzyme and a conversion product thereof, and the second nucleotide coenzyme and a conversion product thereof; and (3) a step of calculating, on the basis of the detected change of the signal, an amount of the kinase forward reaction substrate and/or the phosphorylated product thereof contained in the sample.
A measuring method for at least one of creatine, creatine phosphate, and creatinine, comprising:
Dependencies are inferred from the claim text.