Method for increasing glycosylation of a composition comprising steviol glycosides

A method for increasing the degree of glycosylation of a composition comprising steviol glycosides which method comprises:

• contacting said composition comprising steviol glycosides with a recombinant microorganism, a cell free extract derived from such a recombinant microorganism or an enzyme preparation derived from either thereof wherein the recombinant microorganism is provided in the form of spent biomass from a fermentation process in which steviol glycosides are produced,
• wherein:
• the composition comprising steviol glycosides is a plant extract or a composition of fermentatively produced steviol glycosides;
• the degree of glycosylation of the composition comprising steviol glycosides is increased in the amount of rebaudioside A, rebaudioside D or rebaudioside M present in the composition comprising steviol glycosides;
• and
• the recombinant microorganism comprises one or more nucleotide sequence(s) encoding:
• a polypeptide having ent-copalyl pyrophosphate synthase activity;
• a polypeptide having ent-Kaurene synthase activity;
• a polypeptide having ent-Kaurene oxidase activity;
• a polypeptide having kaurenoic acid 13-hydroxylase activity;
• and
• one or more polypeptides having UDP-glucosyltransferase activity whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least one steviol glycoside.