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US 10,472,660 B2Granted
Method for preparing rebaudioside A from stevioside
AssigneeCj Cheiljedang Corporation
InventorsSung Hee Park, Jung Eun Kim, Ran Young Yoon, Young Ho Hong, Seong Bo Kim, Seung Won Park
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Abstract
The present invention relates to an in-situ method for preparing rebaudioside A from sucrose and stevioside by sucrose synthase and glycosyltransferase.
Claims4
A method for preparing rebaudioside A from stevioside, comprising performing in a single reaction system the following two reactions:
- (1) reacting sucrose and nucleotide diphosphate in the presence of a sucrose synthase to prepare nucleotide diphosphate to which glucose is bonded, and
- (2) reacting the nucleotide diphosphate to which glucose is bonded with stevioside in the presence of a glycosyltransferase to prepare rebaudioside A,
- wherein the sucrose synthase comprises the sequence of SEQ ID NO:3,
- wherein the glycosyltransferase comprises the sequence of SEQ ID NO:4, and
- wherein the reaction system pH is in the range of pH 5.5 to 11.0.
2 more elements
Dependencies are inferred from the claim text.