Isothermal nucleic acid amplification
AssigneeGeneform Technologies Limited
InventorMark Jay Hoser
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Abstract
The present disclosure relates to methods and compositions for ATP regeneration system for use in conjunction with the strand invasion step of an isothermal nucleic acid amplification which uses recombinases that utilize ATP as an energy source.
Claims13
A method of ATP regeneration in a recombinase enzyme reaction comprising providing to the recombinase enzyme reaction phosphocreatine, creatine kinase, sucrose, and sucrose phosphorylase wherein sucrose and sucrose phosphorylase reacts with accumulated orthophosphate.
A composition for ATP regeneration comprising a recombinase, enzymes which enzymatically convert AMP to ADP or ATP, sucrose and sucrose phosphorylase, and phosphocreatine and creatine kinase.
Dependencies are inferred from the claim text.