US 10,472,657 B2Granted

Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes

AssigneeDupont Industrial Biosciences USA, LLC

InventorsKevin D. Nagy, Erwin F. Columbus-Hago, Jayarama K Shetty, Susan Marie Hennessey, Robert Dicosimo, Ling Hua, Rodrigo Ramirez, Zhongmei Tang, Zheyong Yu

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Abstract

A method is disclosed for hydrolyzing an alpha-1,3 or alpha-1,6 glucosyl-glucose linkage in a saccharide (disaccharide or oligosaccharide). This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,3 or alpha-1,6 glucosyl-glucose linkage of the saccharide. This method is useful for reducing the amount of oligosaccharides in a filtrate isolated from a glucan synthesis reaction, for example.

Claims13

Dependent claims

A method that comprises:

(a) providing an alpha-glucan synthesis reaction that produces a composition comprising (i) an alph-glucan product and (ii) a soluble disaccharide or oligosaccharide byproduct, wherein at least 90% of the glycosidic linkages of the alpha-glucan product are alpha-1,3-glycosidic linkages;
and
(b)(1) contacting the soluble disaccharide or oligosaccharide byproduct in said composition with an alpha-glucosidase enzyme, or
(b)(2) obtaining a fraction from said composition, wherein said fraction comprises the soluble disaccharide or oligosaccharide by product, and then contacting the soluble disaccharide or oligosaccharide byproduct in said fraction with an alpha-glucosidase enzyme;
wherein the amount of the soluble disaccharide or oligosaccharide byproduct contacted whith the alpha-glucosidase enzyme in step (b)(1) or (b)(2) is reduced compared to the amount of the soluble disaccharide or oligosaccharide byproduct that was present prior to said contacting.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application: 15/988,349Filed 24 May 2018 · continuation
Priority: WO PCT/CN2015/07326925 Feb 2015
Published: US 2019/0048376 A114 Feb 2019
Granted: 12 Nov 2019
Term ends: Not estimatedThe source records don’t support an estimate.

Classification10 codes

C12P 19/14
Preparation of compounds containing saccharide radicals: produced by the action of a carbohydrase , e.g. by alpha-amylase , e.g. by cellulase,…
C12P 7/06
Acyclic: ethanol, i.e. non-beverage
C12P 7/14
Ethanol, i.e. non-beverage: multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
C12P 19/02
Preparation of compounds containing saccharide radicals: monosaccharides

Family

Application61/945,241Us provisional application · filed 27 Feb 2014
Application61/945,233Us provisional application · filed 27 Feb 2014
Application62/004,290Us provisional application · filed 29 May 2014
Application62/004,300Us provisional application · filed 29 May 2014
Application62/004,305Us provisional application · filed 29 May 2014
Application62/004,308Us provisional application · filed 29 May 2014
Application62/004,312Us provisional application · filed 29 May 2014
Application62/004,314Us provisional application · filed 29 May 2014
Application14/631,962Parent of this continuation · filed 26 Feb 2015
US 2019/0048376 A1Pre-grant publication · published 14 Feb 2019
US 9,988,657 B2Parent of this continuation
US 2015/0240279 A1Published application
US 2017/0314050 A9Published application
US 10,472,657 B2This patent

From the US filing history. Foreign family members aren’t listed.