Enzymes and applications thereof
AssigneeGivaudan SA
InventorsEric Eichhorn, Boris Schilling, Denis Wahler, Laurent Fourage, Esther Locher
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Abstract
There is provided SHC/HAC derivatives, amino acid sequences comprising the SHC/HAC derivatives, nucleotide sequences encoding the SHC/HAC derivatives, vectors comprising nucleotide sequences encoding the SHC/HAC derivatives, recombinant host cells comprising nucleotide sequences encoding the SHC/HAC derivatives and applications of the recombinant host cells comprising either SHC/HAC derivatives or WT SHC/HAC enzymes in methods to prepare (−)-Ambrox and SHC/HAC enzymes in methods to prepare (−)-Ambrox.
Claims25
A process for preparing (−)-Ambrox or a mixture comprising (−)-Ambrox, wherein a mixture of isomers comprising (3E,7E)-homofarnesol (EEH) is enzymatically converted to (−)-Ambrox or a mixture comprising (−)-Ambrox wherein the enzymatic conversion is carried out using an squalene hopene cyclase/homofarnesol Ambrox cyclase (SHC/HAC) enzyme having the polypeptide sequence SEQ ID No. 21 under reaction conditions suitable for the production of (−)-Ambrox, and wherein the mixture of isomers comprises homofarnesol isomers selected from one or more of the following groups consisting of [(3E,7E) and [(3Z,7E)] and/or [(3E,7E) and (3E,7Z)] and/or [(3Z,7E), (3E,7E) and (3E,7Z)] also designated as [EE:EZ], [EE:ZE] and [EE:EZ:ZE] respectively.
Dependencies are inferred from the claim text.