Efficient delivery of large cargos into cells on a porous substrate
AssigneeThe Regents of the University of California
InventorsTing-Hsiang S. Wu, Pei-Yu E. Chiou, Michael A. Teitell
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Abstract
In various embodiments, method and devices for delivering large cargos (e.g., organelles, chromosomes, bacteria, and the like) into cells are provided. In certain embodiments method of delivering a large cargo into eukaryotic cells, are provided that involve providing eukaryotic cells disposed on one side of a porous membrane; providing the cargo to be delivered in a solution disposed in a reservoir chamber on the opposite side of the porous membrane; and applying pressure to the reservoir chamber sufficient to pass the cargo through pores comprising said porous membrane wherein said cargo passes through cell membranes and into the cells.
Claims23
A method of delivering a large cargo into eukaryotic cells, said method comprising:
- providing said cells disposed on one side of a porous membrane that comprises a plurality of pores;
- providing said cargo in a solution disposed in a reservoir chamber on the opposite side of said porous membrane, wherein said cargo ranges in size from 100 nm to 20 μm;
- and
- applying pressure to said reservoir chamber sufficient to pass said cargo through said pores and through the cell membranes of said cells and into said cells to provide cells transfected with said cargo.
Dependencies are inferred from the claim text.