Agents for enhancement of production of biofuel precursors in microalgae
AssigneeThe Regents of the University of California
InventorsChia-Lin Wei, Chew Yee Ngan, Chee Hong Wong, Cindy Choi
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Abstract
We identified 17 transcription factor genes that regulate lipid production and activity in an organism. We subsequently detailed characterization of one of them (psr1). Constructs, methods and systems for enhancing or increasing lipid production in an organism are described.
Claims11
A method for increasing lipid production in a host organism, said method comprising introducing into an organism an expression vector comprising a heterologous nucleotide sequence comprising (a) an operably linked promoter that drives expression in the organism;
- and (b) a lipid regulatory transcription factor gene that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:44;
- culturing the organism under conditions in which the heterologous nucleotide is expressed;
- and selecting an organism that produces increased amounts of lipid compared to wildtype.
A method for enhancing lipid production in a transgenic strain of microalgae, said method comprising introducing into the microalgae an expression cassette comprising a heterologous nucleotide comprising (a) an operably linked promoter that drives expression in the organism;
- and (b) a transcription factor gene from a microalgae that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:44;
- culturing the microalgae under conditions in which the transcription factor gene is expressed;
- and selecting algae that produces increased amounts of lipid compared to wildtype, thereby providing enhanced lipid production in the transgenic strain of microalgae.
Dependencies are inferred from the claim text.