Non-replicative transduction particles and transduction particle-based reporter systems
AssigneeGeneWeave Biosciences, Inc.
InventorsDiego Ariel Rey, Marc Rehfuss, Xiaowen Liu
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Abstract
Methods and systems are provided for packaging reporter nucleic acid molecules into non-replicative transduction particles for use as reporter molecules. The non-replicative transduction particles can be constructed from viruses and use viral transduction and replication systems. The reporter nucleic acid molecules include a reporter gene, such as a reporter molecule or selectable marker, for detecting target genes or cells. Methods and systems are provided for detection of cells and target nucleic acid molecules using the non-replicative transduction particles as reporter molecules.
Claims21
A bacterial cell packaging system for packaging a reporter nucleic acid molecule into a non-replicative transduction particle (NRTP) for introduction into a bacterial cell, the bacterial cell packaging system comprising a host cell, comprising:
- a bacteriophage genome comprising a first terminase gene comprising a disrupted packaging initiation site sequence, wherein in the absence of the disruption the first terminase gene encodes a first terminase protein and wherein the first terminase protein recognizes the first-packaging initiation site sequence, wherein the disruption prevents recognition of the packaging initiation site sequence by the first terminase protein, and wherein the disruption further renders non-functional the first terminase protein;
- and
- a reporter nucleic acid molecule comprising a reporter gene, a first terminase gene comprising a non-disrupted packaging initiation site sequence and encoding a functional first terminase protein, and a second terminase gene encoding a second terminase protein, wherein the non-disrupted packaging initiation site sequence is configured to facilitate packaging of a replicon of the reporter nucleic acid molecule into the NRTP.
Dependencies are inferred from the claim text.