US 10,472,617 B2 · Thermostable asparaginase variants and polynucleotides encoding same

Abstract

The present invention relates to asparaginase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. The invention further relates to a process of producing a fermentation product, comprising: liquefying a starch-containing material to dextrins with an alpha-amylase in the presence of an asparaginase of the invention; saccharifying the dextrins to a sugar with a glucoamylase; and fermenting the sugar using a fermenting organism.

Claims19

Dependent claims

An asparaginase variant comprising

(i) one or more of the following substitutions, wherein each position corresponds to a position in SEQ ID NO:
2:
T71C, T74A, T74C, K122A, K122R, V139G, K194L, D197E, I228M, S238C, N239C, K240R, K253R, I258V, I258Y, R259C, R259V, S297V, S299A, T314A, P333L, S334P, S334W, E337S, S338G, S338W, G356D, K363R or E373H, or
(ii) a deletion at one or more positions corresponding to positions 28 or 29 of SEQ ID NO:
2;
wherein the variant has at least 90% sequence identity to the mature polypeptide of any of SEQ ID NO:
2 or 4, and wherein the variant has asparaginase activity.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application

15/714,292Filed 25 Sep 2017 · division

Priority

EP 1218086117 Aug 2012

Published

US 2018/0030428 A11 Feb 2018

Granted

12 Nov 2019

Term ends

Not estimatedThe source records don’t support an estimate.

Classification6 codes

C12N 9/82
Acting on amide bonds in linear amides: asparaginase
A23L 5/25
Removal of unwanted matter, e.g. deodorisation or detoxification: using enzymes
C12Y 305/01001
In linear amides: asparaginase
C12N
Microorganisms or enzymes

Family

Application61/684,999Us provisional application · filed 20 Aug 2012

PCT/EP2013/067079Parent of this division · filed 15 Aug 2013

US 2018/0030428 A1Pre-grant publication · published 1 Feb 2018

Application14/420,399Parent of this division

US 2015/0210995 A1Published application

US 9,828,595 B2Related patent

US 10,472,617 B2This patent

From the US filing history. Foreign family members aren’t listed.

Thermostable asparaginase variants and polynucleotides encoding same

Life of this patent

Thermostable asparaginase variants and polynucleotides encoding same

Life of this patent

Abstract

Claims19