Genes encoding alpha-1,3-glucanase and methods of using the same
AssigneeGenofocus Co., Ltd.
InventorsJae Gu Pan, Eui Joong Kim, Taek Ho Yang, Dong Beom Lee, Eun Young Kim, Hye Rim Lee, Jung Hyun Kang
Life of this patent
Estimating the term…
Abstract
The present invention relates to a novel alpha-1,3-glucanase, and more particularly, to a novel alpha-1,3-glucanase from Trichoderma harzianum, a gene encoding the same, a recombinant vector and recombinant microorganism comprising the gene, and a method of producing an alpha-1,3-glucanase using the recombinant microorganism. The novel alpha-1,3-glucanase according to the present invention can effectively degrade mutan, which is a component of a microbial biofilm, and thus it can be used in oral hygiene products and the medical field.
Claims5
A recombinant Aspergillus niger microorganism transformed with a pASP vector, the vector comprising a nucleotide sequence of SEQ ID NO:
- 3, wherein the recombinant Aspergillus niger microorganism produces a secreted glycosylated alpha-1,3-glucanase comprising the amino acid sequence of SEQ ID NO:
- 1, wherein the secreted glycosylated alpha-1,3-glucanase has a molecular weight in a range of 115 to 240 kDa, and wherein the secreted glycosylated alpha-1,3-glucanase has enzymatic activity against mutan produced by Streptococcus mutans.
Dependencies are inferred from the claim text.