Back to results
US 10,472,606 B2Granted
Cell preservation method for pluripotent stem cells
AssigneeES Cell International PTE Ltd.
InventorsJeremy M. Crook, Lucy Kravets
Life of this patent
Estimating the term…
Abstract
The present invention provides a method for freezing a stem cell or a cell derived therefrom, the method including the steps of providing a cell suspension, performing ice nucleation on the cell suspension, and lowering the temperature of the ice nucleated cell suspension to a temperature sufficiently low to allow long term storage of the stem cell. The method is preferably used for the cryopreservation of human embryonic stem cells.
Claims19
A composition comprising:
- (a) adenosine, dextran-40, lactobionic acid, HEPES (N-(2-Hydroxyethyl) piperazine-N′-(2-ethanesulfonic acid)), sodium hydroxide, L-glutathione, potassium chloride, potassium bicarbonate, potassium phosphate, dextrose, sucrose, mannitol, calcium chloride, magnesium chloride, potassium hydroxide, sodium hydroxide, dimethyl sulfoxide (DMSO), and water;
- and
- (b) pluripotent stem cells, wherein the composition can be stored at a temperature sufficiently low enough to allow long term storage of the pluripotent stem cells.
Dependencies are inferred from the claim text.