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US 10,472,605 B2Granted
Serum-free medium
AssigneeNational University of Ireland, Galway
InventorsFrancis Peter Barry, Emma Jane Mooney, Josephine Mary Murphy, Georgina Margaret Shaw, Sean Patrick Gaynard
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Abstract
A serum-free medium for the growth of mesenchymal stem cells comprises FGF, TGF-β and lipoprotein.
Claims15
A serum-free medium for culturing mesenchymal stem cells, comprising:
- (i) as growth factors,
- (a) fibroblast growth factor (FGF) and (b) transforming growth factor beta (TGFβ), and no other growth factors;
- and
- (ii) a basal medium supplemented with lipoprotein, insulin-transferrin-selenium (ITS), ascorbic acid, dexamethasone, human serum albumin, penicillin and streptomycin.
1 more element
A serum-free medium for culturing mesenchymal stem cells consisting of:
- fibroblast growth factor (FGF);
- transforming growth factor beta (TGF-β);
- and
- a basal medium supplemented with lipoprotein, insulin-transferrin-selenium (ITS), ascorbic acid, dexamethasone, human serum albumin, penicillin and streptomycin.
1 more element
Dependencies are inferred from the claim text.