Back to results
US 10,472,409 B2Granted
Methods of making high-strength NDGA polymerized collagen fibers and related collagen-prep methods, medical devices and constructs
AssigneeShriners Hospitals for Children
InventorsThomas J. Koob, Douglas Pringle, Daniel Hernandez
Life of this patent
Estimating the term…
Abstract
The disclosure describes methods of making high-strength NDGA collagen and associated methods of preparing collagen preparatory material and medical bioprostheses.
Claims15
A method of manufacturing nordihydroguaiaretic acid (NDGA) polymerized collagen fibers, comprising:
- (a) treating collagen with a solution comprising NDGA to provide NDGA-treated collagen;
- (b) drying the NDGA-treated collagen while holding the NDGA-treated collagen in tension for a period of time;
- (c) washing the dried NDGA-treated collagen in a solution to remove unreacted soluble NDGA cross-linking intermediates;
- (d) drying the NDGA-treated collagen while holding the NDGA-treated collagen in tension for a period of time;
- and
- repeating steps (a)-(d) at least once to produce high-strength NDGA polymerized collagen fibers,
- wherein the high-strength NDGA polymerized collagen fibers have an average tensile strength of at least about 150 MPa.
4 more elements
A method of organizing collagen before cross-linking, comprising:
- purifying donor collagen preparatory material;
- dialyzing the purified collagen preparatory material at least three times against deionized water in a volume ratio of between about 30:1 to about 100:1, for between about 30-90 minutes to form a dialyzed collagen material;
- and
- forming a substantially clear gel using the dialyzed collagen material thereby indicating improved organization of collagen fibrils.
1 more element
Dependencies are inferred from the claim text.