Fusion proteins comprising partial tetraspanin sequences and a system thereof for presenting peptides on the cell surface
AssigneePeter Und Traudl Engelhorn-Stiftung Zur FöRderung Der Lebenswissenschaften
InventorDaniel Ivanusic
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Abstract
The subject-matter of the present application concerns fusion proteins based on tetraspanin proteins, in which the extracellular loop is replaced in whole or in part by peptide sequences of a different composition and also their production and use as transport vehicle. The disclosed fusion proteins are anchored in the membrane of cells and can be used to present foreign peptides fused with them on the cell surface.
Claims28
A fusion protein comprising a first domain (i), a second domain (ii) and a third domain (iii), wherein the second domain is disposed between the first and the third domain, wherein said first (i), second (ii) and third domain (iii) are as follows:
- (i) a partial sequence of a tetraspanin, which includes the transmembrane domain 1 (TM1), the small extracellular loop (SEL), the transmembrane domain 2 (TM2), the small intracellular loop (SIL) and the transmembrane domain 3 (TM3), or comprises a sequence homologous thereto with a sequence identity of at least 95% over the entire length,
- (ii) a peptide with a predetermined amino acid sequence having a sequence identity of less than 70% over the entire length with respect to the large extracellular loop (LEL) of a tetraspanin and
- (iii) a partial sequence of a tetraspanin, comprising the transmembrane domain 4 (TM4) or a sequence homologous thereto with a sequence identity of at least 95% over the entire length,
- wherein the second domain (ii) further comprises one or more protease recognition sequences, which are disposed C- and/or N-terminally to the peptide with the predetermined amino acid sequence.
A nucleic acid molecule comprising a first sequence segment (i), a second sequence segment (ii) and a third sequence segment (iii), wherein the second sequence segment is disposed between the first and the third sequence segment, wherein said first (i), second (ii) and third (iii) sequence segment are as follows:
- (i) nucleic acid molecule codes for a partial sequence of a tetraspanin, which includes the transmembrane domain 1 (TM1), the small extracellular loop (SEL), the transmembrane domain 2 (TM2), the small intracellular loop (SIL) and the transmembrane domain 3 (TM3), or a sequence homologous thereto with a sequence identity of at least 95% over the entire length,
- (ii) nucleic acid molecule comprises a multiple cloning site and
- (iii) nucleic acid molecule codes for a partial sequence of a tetraspanin, comprising the transmembrane domain 4 (TM4) or a sequence homologous thereto with a sequence identity of at least 95% over the entire length,
- wherein the second nucleic acid molecule (ii) further comprises one or more protease recognition sequences, which are disposed C- and/or N-terminally to the multiple cloning site.
Dependencies are inferred from the claim text.