Use of Cyt1Aa as broad spectrum targeting domain for insecticidal proteins with basic research and biotechnology applications

Abstract

A chimeric protein comprising a cytolytic δ-endotoxin portion and a heterologous polypeptide portion is provided. Nucleic acids encoding the chimeric protein are also provided. In some cases, the chimeric protein can be expressed in parasporal inclusions of Bacillus thuringiensis. In certain cases, the cytolytic δ-endotoxin portion can include Cyt1Aa, and the heterologous portion can include an insecticidal polypeptide. The chimeric protein can be expressed in a suitable B. thuringiensi cell and purified as parasporal inclusions. Also provided is a method of controlling insects by exposing an insect to a chimerical insecticidal protein.

Dependencies are inferred from the claim text.