Modular base-specific nucleic acid binding domains from burkholderia rhizoxinica proteins
AssigneeCellectis
InventorsClaudia Bertonati, Philippe Duchateau, Alexandre Juillerat, George Silva, Julien Valton
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Abstract
The present invention concerns new modular base-per-base specific nucleic acid binding domains (MBBBD) derived from newly identified proteins from the bacterial endosymbiont Burkholderia Rhizoxinica and their use for engineering nucleic acid processing enzymes, such as specific endonucleases or transcription activators.
Claims13
A nucleic acid binding polypeptide that comprises a succession of at least 12 modules, wherein each module consists of 30 to 35 contiguous amino acid residues, wherein said succession of modules displays a base per base specificity towards a nucleic acid target sequence,
- wherein at least one of said modules has at least 70% sequence identity with one of the module polypeptide sequences from proteins E5AV36, E5AW43 and E5AW45 of Burkholderia rhizoxinica;
- wherein said nucleic acid binding polypeptide is fused to a catalytic domain from I-Tevl, and
- wherein said polypeptide binds to and cleaves the nucleic acid target sequence on a chromosome in a mammalian cell.
Dependencies are inferred from the claim text.