Substrate for supporting liquid sample, an assembly comprising such a substrate and use thereof
AssigneeElvesys
InventorsMaël Le Berre, Adrien Plecis
Life of this patent
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Abstract
A micro-structured substrate to support a liquid sample having a lower face and an upper face. At least one surface cavity of volume V0 opening onto the upper face and forming a zone for analyzing the liquid sample. A first groove having a first volume V1, positioned around each surface cavity and opening onto the upper face thereof. The substrate also includes at least one second groove opening onto the upper face thereof and positioned around the first groove. The sum of the volumes of the second grooves is equal to V2. The volume V1+V2 is greater than or equal to 0.05 V0, preferably 0.1 V0. An analysis assembly including the substrate and the use thereof is also contemplated herein.
Claims21
A micro-structured substrate having a structure to support a liquid sample, comprising:
- a lower face;
- an upper face;
- at least one surface cavity, having a volume V0, opening onto said upper face and forming an analysis zone for the liquid sample;
- a first groove, having a first volume V1, arranged around each surface cavity and opening onto said upper face;
- at least one second groove opening on said upper face and arranged around the first groove;
- wherein a sum of volumes of the second grooves being equal to V2, a volume V1+V2 being greater than or equal to 0.05 V0, a depth of the first and second grooves being between 10 and 500 microns;
- wherein the first and second grooves are continuous and not in a fluid communication with one another;
- and
- wherein the structure of the micro-structured substrate prevents generation of bubbles when closing the cavities with a cover slip.
An optical analysis assembly for a liquid sample, comprising:
- a micro-structured substrate having a structure to support the liquid sample, the substrate comprising,
- a lower face;
- an upper face;
- at least one surface cavity, having a volume V0, opening onto said upper face and forming an analysis zone for the liquid sample;
- a first groove, having a first volume V1, arranged around each surface cavity and opening onto said upper face;
- at least one second groove opening on said upper face and arranged around the first groove;
- wherein a sum of volumes of the second grooves being equal to V2, a volume V1+V2 being greater than or equal to 0.05 V0, a depth of the first and second grooves being between 10 and 500 microns;
- and
- wherein the first and second grooves are continuous and not in a fluid communication with one another;
- at least one liquid sample arranged in said at least one surface cavity of the micro-structured substrate, a volume of the liquid sample being greater than a volume of said at least one surface cavity on which said at last one liquid sample is arranged;
- a cover slip covering the micro-structured substrate and said at least one liquid sample, closing all the cavities of the micro-structured substrate;
- and
- wherein the structure of the micro-structured substrate prevents generation of bubbles when closing the cavities with the cover slip.
Dependencies are inferred from the claim text.