High-density microchamber array and manufacturing method thereof

A high-density micro-chamber array, comprising:

• a first micro-chamber member comprising:
• a flat first substrate;
• a plurality of first micro-chambers, each having a capacity of not greater than 4000×10−18 m3, that are formed from a hydrophobic first material, and are arrayed regularly at a density of about 2×106 per 1 cm2 on a surface of the first substrate;
• and
• a first lipid membrane that is formed at openings of the plurality of first micro-chambers filled with a first aqueous test solution to liquid-seal the first aqueous test solution;
• and
• a second micro-chamber member comprising:
• a flat second substrate;
• a plurality of second micro-chambers, each having a capacity of not greater than 4000×10−18 m3, that are formed from a hydrophobic second material, and are arrayed regularly at density of about 2×106 per 1 cm2 on a surface of the second substrate;
• and
• a second lipid membrane that is formed at openings of the plurality of second micro-chambers filled with a second aqueous test solution to liquid-seal the second aqueous test solution, wherein
• the first micro-chamber member and the second micro-chamber member are joined with each other such that the first lipid membrane and the second lipid membrane are stacked and configured as a lipid bilayer membrane.