Bacteriophage-polymer hybrid
AssigneeImperial College Innovations Limited
InventorsAmin Hajitou, Teerapong Yata
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Abstract
The invention provides a targeted bacteriophage-polymer complex comprising a recombinant targeted-bacteriophage and a cationic polymer. The complex has a net positive charge. The invention provides methods of preparing bacteriophages and complexes thereof, and to their uses for the delivery of transgenes in a variety of gene therapy applications.
Claims18
A method of delivering a transgene to a mammalian cell, comprising:
- administering to a mammalian cell a targeted bacteriophage-polymer complex comprising a recombinant negatively charged targeted-bacteriophage and a cationic polymer selected from the group consisting of poly-D-lysine (PDL), diethylaminoethyl-dextran (DEAE.DEX) and polyethyleneimine (PEI), wherein the recombinant bacteriophage comprises a nucleic acid sequence, which encodes a protein ligand that is capable of being expressed on the capsid coat of the recombinant bacteriophage, and which is specific for a protein expressed on a mammalian target cell or tissue, so as to enable targeted delivery thereto, wherein the complex has a net positive charge and a ζ-potential of at least 5 mV at physiological pH, and wherein the complex comprises a weight:
- weight ratio of about 100 ng to 400 ng polymer:1 μg phage.
A method of delivering a transgene to a mammalian cell, comprising:
- administering to a mammalian cell an aggregate comprising a plurality of bacteriophage-polymer complexes comprising a recombinant negatively charged targeted-bacteriophage and a cationic polymer selected from the group consisting of poly D-lysine (PDL), diethylaminoethyl-dextran (DEAE.DEX) and polyethyleneimine (PEI), wherein the recombinant bacteriophage comprises a nucleic acid sequence, which encodes a protein ligand that is capable of being expressed on the capsid coat of the recombinant bacteriophage, and which is specific for a protein expressed on a mammalian target cell or tissue, so as to enable targeted delivery thereto, wherein the complex has a net positive charge and a ζ-potential of at least 5 mV at physiological pH, and wherein the complex comprises a weight:weight ratio of about 100 ng to 400 ng polymer:1 μg phage.
A targeted bacteriophage-polymer complex comprising a recombinant negatively charged targeted-bacteriophage and a cationic polymer selected from the group consisting of poly-D-lysine (PDL), diethylaminoethyl-dextran (DEAE.DEX) and polyethyleneimine (PEI), wherein the recombinant bacteriophage comprises a nucleic acid sequence, which encodes a protein ligand that is capable of being expressed on the capsid coat of the recombinant bacteriophage, and which is specific for a protein expressed on a mammalian target cell or tissue, so as to enable targeted delivery thereto, wherein the complex has a net positive charge and a ζ-potential of at least 5 mV at physiological pH, and wherein the complex comprises a weight:
- weight ratio of about 100 ng to 400 ng polymer:1 μg phage.
Dependencies are inferred from the claim text.