Management of ischemia using pooled mesenchymal stromal cell composition

A method of treating ischemia in a subject having or suspected of having the ischemia, said method comprising acts of administering a composition comprising pooled and expanded allogeneic mesenchymal stromal cells at a dose ranging from about 0.5 million cells per kg of body weight of the subject to 5 million cells per kg of body weight of the subject, optionally along with pharmaceutically acceptable excipient to the subject, wherein said composition is administered intramuscularly through a combination of at least two sites selected from the group comprising calf muscle region, thigh region, at or along or in the course of a constricted vessel, locally at or near a site affected by the ischemia and local administration at or around the site of an ulcer, gangrene, or wound;

• and
• topical administration of a conditioned medium to the site affected by the ischemia, and wherein said administration of the conditioned medium is carried out either simultaneously or sequentially with the administration of the composition;
• wherein the conditioned medium is derived from the pooled and expanded allogeneic mesenchymal stromal cells.