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US 10,470,798 B1Granted
Methods for promoting fertilization
AssigneeOhana Biosciences, Inc.
InventorsFelipe A. Navarrete Solano, Eric Steven Furfine, Kathleen Inez Seyb
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Abstract
The disclosure provides, inter alia, methods of improving sperm function and related methods of fertilization, together with preparations of activated or potentiated sperm. The disclosure additionally provides articles of manufacture suitable for performing the methods provided by the invention. The methods provided by the disclosure, in some embodiments entail energy depletion with subsequent staged reintroduction of different energy sources.
Claims20
A method for promoting fertilization comprising:
- (a) incubating a mammalian sperm under energy depletion conditions for a time suitable to potentiate the mammalian sperm;
- (b) providing the potentiated mammalian sperm from step (a) with an effective amount of a first energy source and a second energy source in a serial manner;
- and
- (c) providing the mammalian sperm resulting from step (b) with access to an egg under conditions to promote fertilization, wherein the effective amount is an amount sufficient to induce improved sperm function.
1 more element
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