Electron consuming ethanol production pathway to displace glycerol formation in S. cerevisiae
AssigneeLallemand Hungary Liquidity Management LLC
InventorsArthur J. Shaw, IV, Aaron Argyros, Trisha Barrett
Life of this patent
Estimating the term…
Abstract
The present invention provides for a mechanism to completely replace the electron accepting function of glycerol formation with an alternative pathway to ethanol formation, thereby reducing glycerol production and increasing ethanol production. In some embodiments, the invention provides for a recombinant microorganism comprising a down-regulation in one or more native enzymes in the glycerol-production pathway. In some embodiments, the invention provides for a recombinant microorganism comprising an up-regulation in one or more enzymes in the ethanol-production pathway.
Claims80
A co-culture comprising at least two host cells wherein
- one of the host cells is a recombinant microorganism and comprises:
- (i) a heterologous nucleic acid encoding a phosphoketolase;
- (ii) at least one heterologous nucleic acid encoding an enzyme in an acetyl-CoA production pathway;
- (iii) a heterologous nucleic acid encoding a bifunctional acetaldehyde-alcohol dehydrogenase;
- and,
- (iv) at least one genetic modification that leads to the down-regulation of an enzyme in a glycerol-production pathway.
A co-culture comprising at least two host cells wherein one of the host cells is a recombinant microorganism and comprises:
- (i) at least one heterologous nucleic acid encoding an enzyme in an acetyl-CoA production pathway;
- (ii) a heterologous nucleic acid encoding a bifunctional acetaldehyde-alcohol dehydrogenase;
- and,
- (iii) at least one genetic modification that leads to the down-regulation of an enzyme in a glycerol-production pathway.
Dependencies are inferred from the claim text.