Antibodies to quetiapine haptens and use thereof
AssigneeJanssen Pharmaceutica NV
InventorsEric Hryhorenko, Banumathi Sankaran, Thomas R. DeCory, Theresa Tubbs, Linda Colt, Bart M. Remmerie, Rhys Salter, Matthew Garrett Donahue, Yong Gong
Life of this patent
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Abstract
Disclosed is an antibody which binds to quetiapine, which can be used to detect quetiapine in a sample such as in a competitive immunoassay method. The antibody can be used in a lateral flow assay device for point-of-care detection of quetiapine, including multiplex detection of aripiprazole, olanzapine, quetiapine, and risperidone in a single lateral flow assay device.
Claims14
A method of producing an antibody or a binding fragment thereof which specifically binds to quetiapine, the method comprising:
- (i) selecting a host for antibody production;
- and
- (ii) inoculating the host with a conjugate of a compound of Formula I and an immunogenic carrier;
- and
- (iii) isolating an antibody or binding fragment thereof which specifically binds to quetiapine produced by the host,
- Formula I:
- wherein:
- R1 is H,
- CH2NH2, or CH2NHC(O)(CH2)mCO2H;
- R2 is H,
- CH2NH2, or CH2NHC(O)(CH2)mCO2H;
- R3 is H;
- provided that either R1 or R2 must be H, and further provided that both R1 and R2 may not be H simultaneously;
- m is 1, 2, 3, 4, or 5;
- and
- n is 1, 2, 3, 4, or 5, and wherein the immunogenic carrier is conjugated to the compound of Formula I at R1 when R1 is not H, or at R2 when R2 is not H.
A method of producing a hybridoma cell line capable of producing a monoclonal antibody which binds to quetiapine, the method comprising:
- (i) selecting a host for antibody production;
- (ii) inoculating the host with a conjugate of a compound of Formula I and an immunogenic carrier;
- (iii) fusing a cell line capable of producing an antibody that binds to quetiapine from said inoculated host with an immortalized cell to create a fused cell capable of producing a monoclonal antibody which binds to quetiapine;
- and
- (iv) cloning the fused cell so as to obtain a hybridoma cell line, Formula I:
- wherein:
- CH2NH2, or CH2NHC(O)(CH2)mCO2H;
- R2 is H,
- CH2NH2, or CH2NHC(O)(CH2)mCO2H;
- R3 is H;
- provided that either R1 or R2 must be H, and further provided that both R1 and R2 may not be H simultaneously;
- m is 1, 2, 3, 4, or 5;
- and
- n is 1, 2, 3, 4, or 5, and wherein the immunogenic carrier is conjugated to the compound of Formula I at R1 when R1 is not H, or at R2 when R2 is not H.
Dependencies are inferred from the claim text.