Neurospora crassa strains with amplified expression of cellulases and production of biofuel therefrom
AssigneeUniversity of Cincinnati
InventorsChristian Hong, Toru Matsuura
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Abstract
Transgenic strains of Neurospora crassa engineered to comprise a synthetic positive feedback loop for a transcriptional activator of cellulase expression such that cellulase production is amplified are disclosed, along with compositions thereof. The transgenic strains are particularly useful in methods for generating purified cellulases, fermentable sugars, and cellulosic ethanol for the efficient production of biofuel from cellulose-containing biomass and waste.
Claims16
A transgenic strain of Neurospora crassa engineered to comprise a synthetic positive feedback loop for a transcriptional activator of cellulase expression encoded by N. crassa clr-1 gene or N. crassa clr-2 gene, wherein the strain has been genetically modified by operably linking a promoter of a gene encoding cellobiohydrolase-1 (Pcbh-1) to the N. crassa clr-1 or N. crassa clr-2 genes.
A method for increasing expression of at least one cellulase in an N. crassa strain, wherein said method comprises engineering an N. crassa strain to comprise a synthetic positive feedback loop for a transcriptional activator of cellulase expression encoded by N. crassa clr-1 gene or N. crassa clr-2 gene, wherein the strain has been genetically modified by operably linking a promoter of a gene encoding cellobiohydrolase-1 (Pcbh-1) to the N. crassa clr-1 or N. crassa clr-2 genes.
Dependencies are inferred from the claim text.