Microorganisms and methods for producing biologics and introducing biologics to sites
AssigneeWisconsin Alumni Research Foundation
InventorsJan Peter Van Pijkeren, Jee-Hwan Oh
Life of this patent
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Abstract
Microorganisms comprising a maltose-inducible promoter and methods of use in producing biologics and introducing biologics to sites in a maltose-dependent manner. The microorganisms include a maltose-inducible promoter operably connected to a coding sequence of a biologic. The biologic may be a polypeptide or a nucleic acid. Polypeptide biologics may include lytic proteins and/or secreted proteins. Nucleic acid biologics may include antisense RNA, other types of RNA, or other types of nucleic acids. The microorganisms can be used to produce the biologics and/or introduce the biologics to in vitro or in vivo sites in a maltose-dependent manner. The microorganisms can also be used in maltose-dependent gene silencing.
Claims19
A method of introducing a biologic to a site, the method comprising introducing a microorganism harboring a recombinant gene to the site, wherein the recombinant gene comprises a maltose-inducible promoter operably connected to a coding sequence of a first biologic, wherein the maltose-inducible promoter is at least about 90% identical to the sequence of SEQ ID NO:1, wherein expression of the first biologic from the coding sequence effects release of the first biologic from the microorganism at the site.
A method of producing a biologic, the method comprising exposing a microorganism to an amount of maltose, wherein the microorganism harbors a recombinant gene, wherein the recombinant gene comprises a maltose-inducible promoter operably connected to a coding sequence of a first biologic, wherein the maltose-inducible promoter is at least about 90% identical to the sequence of SEQ ID NO:1, wherein the amount of maltose is sufficient to stimulate production of the first biologic from the coding sequence.
Dependencies are inferred from the claim text.