Tobacco plants exhibiting altered photosynthesis and methods of making and using
AssigneeAltria Client Services LLC
InventorsYanxin Shen, Dongmei Xu
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Abstract
This disclosure provides tobacco plants that exhibit altered photosynthesis as well as methods of making and using such plants.
Claims21
A method of making a Nicotiana tabacum plant, comprising:
- inducing mutagenesis in N. tabacum cells to produce mutagenized N. tabacum cells;
- obtaining one or more N. tabacum plants from said mutagenized N. tabacum cells;
- and
- identifying at least one of said N. tabacum plants that comprises a mutation in a sequence having at least 95% sequence identity to SEQ ID NO:15 or 17.
A Nicotiana tabacum plant, or part thereof, comprising a mutation in an endogenous nucleic acid, the wild type endogenous nucleic acid encoding a sequence having at least 95% sequence identity to a sequence selected from the group consisting of SEQ ID NOs:
- 16 and 18.
A nucleic acid molecule comprising a first nucleic acid sequence between 15 and 500 nucleotides in length and a second nucleic acid sequence between 15 and 500 nucleotides in length, wherein the first nucleic acid sequence has a region of complementarity comprising at least 15 contiguous nucleotides that are complementary to the second nucleic acid sequence, wherein said region of complementarity of the first nucleic acid sequence comprises at least 15 contiguous nucleotides of the sequence shown in SEQ ID NO:
- 15 or 17.
A transgenic Nicotiana tabacum plant comprising a vector, a vector comprising a nucleic acid sequence having a length of 15 to 500 nucleotides and having at least 95% sequence identity to the complement of SEQ ID NO:
- 15 or 17;
- wherein the expression of an endogenous gene comprising SEQ ID NO:15 or 17 is reduced relative to a control plant lacking said vector.
Dependencies are inferred from the claim text.