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US 10,450,616 B1Granted
Polynucleotides for the amplification and detection of Chlamydia trachomatis
AssigneeTalis Biomedical Corporation
InventorsAndrea Dedent, Matt Lee, Shuyuan Ma, Hédia Maamar
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Abstract
The invention provides methods and compositions for the detection of Chlamydia trachomatis in a test sample. Its presence or absence in the sample is determined by nucleic acid based testing methods using primers and/or probes and or molecular beacons that bind to the 23S ribosomal genes or gene transcripts.
Claims27
A composition comprising a set of primers selected from the group consisting of:
- i. a primer set comprising a forward outer primer comprising SEQ ID NO:
- 87, a backward outer primer comprising SEQ ID NO:
- 82, a forward inner primer comprising SEQ ID NO:
- 94, a backward inner primer comprising SEQ ID NO:
- 84, a forward loop primer comprising SEQ ID NO:
- 76, and a backward loop primer comprising SEQ ID NO:
- 86;
- ii. a primer set comprising a forward outer primer comprising SEQ ID NO:
- 87, a backward outer primer comprising SEQ ID NO:
- 82, a forward inner primer comprising SEQ ID NO:
- 94, and a backward inner primer comprising SEQ ID NO:
- 84;
- and
- iii. a primer set comprising a forward inner primer comprising SEQ ID NO:
- 94 and a backward inner primer comprising SEQ ID NO:
- 84.
13 more elements
A method of detecting Chlamydia trachomatis in a test sample, the method comprising:
- (a) extracting nucleic acid from the test sample;
- (b) amplifying a target sequence by reacting the nucleic acid extracted in step (a) with a reaction mixture comprising a strand displacement DNA polymerase and a sequence-specific primer set, wherein said sequence-specific primer set is selected from the group consisting of:
- (i) a primer set comprising a forward outer primer comprising SEQ ID NO:
- 87, a backward outer primer comprising SEQ ID NO:
- 82, a forward inner primer comprising SEQ ID NO:
- 94, a backward inner primer comprising SEQ ID NO:
- 84, a forward loop primer comprising SEQ ID NO:
- 76, and a backward loop primer comprising SEQ ID NO:
- 86;
- (ii) a primer set comprising a forward outer primer comprising SEQ ID NO:
- 87, a backward outer primer comprising SEQ ID NO:
- 82, a forward inner primer comprising SEQ ID NO:
- 94, and a backward inner primer comprising SEQ ID NO:
- 84;
- and
- (iii) a primer set comprising a forward inner primer comprising SEQ ID NO:
- 94 and a backward inner primer comprising SEQ ID NO:
- 84;
- and
- (c) detecting the presence or absence of an amplified product of step (b);
- wherein the presence of said amplification product is indicative of the presence of Chlamydia trachomatis in the test sample.
18 more elements
A method of detecting Chlamydia trachomatis in a test sample, the method comprising:
- (a) extracting nucleic acid from the test sample;
- (b) amplifying a target sequence by reacting the nucleic acid extracted in step (a) for less than ten minutes with a reaction mixture comprising a strand displacement DNA polymerase and a sequence-specific LAMP primer set comprising a forward outer primer comprising SEQ ID NO:
- 87, a backward outer primer comprising SEQ ID NO:
- 82, a forward inner primer comprising SEQ ID NO:
- 94, a backward inner primer comprising SEQ ID NO:
- 84, a forward loop primer comprising SEQ ID NO:
- 76, and a backward loop primer comprising SEQ ID NO:
- 86;
- and
- (c) detecting the presence or absence of an amplified product of step (b);
- wherein the presence of said amplification product is indicative of the presence of Chlamydia trachomatis in the test sample.
8 more elements
Dependencies are inferred from the claim text.