Root-preferred promoter from a Panicum virgatum metallothionein-like gene
AssigneeDow AgroSciences LLC
InventorsSandeep Kumar, Daren Hemingway, Carla Ausmus, Andrew F. Worden, Andrew Asberry
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Abstract
This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Panicum virgatum metallothionein-like gene (mtl). Some embodiments relate to a promoter from a Panicum virgatum metallothionein-like gene (mtl) that functions in plants to promote transcription of operably linked nucleotide sequences.
Claims18
A gene expression cassette comprising a promoter operably linked to a heterologous nucleic acid, wherein the promoter comprises a polynucleotide comprising a sequence identity of at least 97% to SEQ ID NO:1, and said promoter has below ground tissue preferred expression.
A method for expressing a coding sequence in a transgenic plant, the method comprising:
- a) transforming a plant cell with a gene expression cassette comprising a polynucleotide sequence comprising a sequence identity of at least 97% to SEQ ID NO:1 operably linked to a heterologous coding sequence, which is operably linked to a 3′ untranslated region;
- b) isolating the transformed plant cell comprising the gene expression cassette;
- c) regenerating a transgenic plant from the transformed plant cell;
- and,
- d) obtaining the transgenic plant, wherein the transgenic plant expresses the coding sequence with below ground tissue preferred expression.
A method for manufacturing a synthetic polynucleotide sequence comprising a sequence identity of at least 97% to SEQ ID NO:1, the method comprising:
- a) isolating a nucleic acid comprising a polynucleotide sequence comprising SEQ ID NO:1;
- b) producing a plurality of oligonucleotide primer sequences, wherein the oligonucleotide primer sequences bind to the nucleic acid under stringent hybridization conditions;
- c) ligating the plurality of oligonucleotide primer sequences to synthesize a synthetic polynucleotide sequence;
- and,
- d) sequencing the resulting synthetic polynucleotide to confirm that it comprises at least 97% identity to SEQ ID NO:1.
Dependencies are inferred from the claim text.