Methods of producing viruses
AssigneeBenchmark Animal Health Limited
InventorsLindsey Ann Toon, Ralf Hoffmann
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Abstract
Methods of producing, propagating and multiplying viruses, methods of identifying the presence of a virus in a sample, specifically methods using Japanese eel cell cultures. Also provided are an isolated Japanese eel cell culture, optionally infected with a virus, vaccines comprising viruses grown according to the methods of the invention, and a kit for propagating viruses comprising the Japanese eel cell culture. The virus may be a fish virus such as nodavirus, Infectious Pancreatic Necrosis Virus (IPNV) and Salmon Pancreas Disease Virus (SPDV).
Claims11
A method of propagating a virus comprising the steps:
- infecting a cell culture with the virus;
- culturing the infected cell culture;
- harvesting the virus;
- and
- optionally purifying the virus,
- wherein said cell culture is the cell culture Designated Anguilla japonica-K deposited with accession number 16062701.
An isolated Japanese eel cell from the cell culture designated Anguilla japonica-K deposited with accession number 16062701 infected with a virus.
An isolated cell culture designated Anguilla japonica-K deposited with accession number ECACC 16062701.
Dependencies are inferred from the claim text.