US 10,444,236 B2 · Method of detection of proteolysis products in plasma and a diagnostic system for its application

Abstract

Immunological diagnostic methods that use the whole-length molecule of plasminogen or its peptide fragments as universal detectors of proteolysis products having a C-terminal lysine are proposed. The method of this immunological diagnostic is to identify the human diseases associated with increased activity of proteolytic enzymes. A diagnostic test system comprised of a detector—the full-length molecule and its presented peptide fragments—is disclosed. The technical result includes achieving the required degree of dissociation of the antigen-antibody complex in a sample from the subject, as well as changing the conformation of proteins using an incubation buffer containing organic solvents in the disclosed ratios, that can significantly increase the sensitivity of the method for determining the concentration of proteolytic fragments with a C-terminal lysine binding with plasminogen or fragments thereof.

Claims14

Dependent claims

A method for identifying a subject having an increased blood plasma concentration of proteolytic products having a C-terminal lysine, comprising the following steps:

providing a human blood plasma sample obtained from a subject;
contacting the blood plasma sample or a component thereof with a fragment of plasminogen in the presence of a buffer composition, wherein said fragment of plasminogen consists of an amino acid sequence of SEQ ID NO:
1 or SEQ ID NO:
2 or SEQ ID NO:
3 or SEQ ID NO:
4, and
detecting complexes comprising the fragment of plasminogen bound to the proteolytic product having a C-terminal lysine;
and measuring the level of proteolytic products having a C-terminal lysine in the sample;
wherein an increased amount of complexes or an increased level of proteolytic products having a C-terminal lysine in the sample relative to a control sample is indicative of said human having an increased blood plasma concentration of proteolytic products having a C-terminal lysine.

6 more elements

Dependencies are inferred from the claim text.

Method of detection of proteolysis products in plasma and a diagnostic system for its application

Life of this patent

Method of detection of proteolysis products in plasma and a diagnostic system for its application

Life of this patent

Abstract

Claims14