Fluorous oligonucleotide microarray

A microarray for detecting targets and biomolecule interactions, comprising:

• (a) a covalently-modified electrically conductive fluorous surface comprising a compositional grid,
• (i) wherein the surface is electrically conductive with a sheet resistivity of less than 50 ohms/square, and
• (ii) wherein the electrically conductive surface is perfluorocarbon modified;
• (b) a plurality of fluorous-modified oligonucleotide compositions, wherein each fluorous-modified oligonucleotide composition in the plurality of fluorous-modified oligonucleotide compositions includes:
• (i) a terminal fluorous domain or tag comprising at least one perfluoroalkyl group in the fluorous domain or tag, wherein the terminal fluorous domain or tag immobilizes each fluorous-modified oligonucleotide composition on the perfluorocarbon covalently-modified electrically conductive surface by non-covalently interacting with the perfluorocarbon modified surface through fluorous partitioning, wherein a fluorous-modified oligonucleotide composition occupies a defined location within the grid;
• (ii) a linker connected to the terminal fluorous domain or tag, wherein the linker is a diamine linker, a phosphonic acid-hydroxyl linker, or an alkynyl amine linker;
• and
• (iii) an oligonucleotide connected to the linker, wherein the oligonucleotide is configured to bind targets and interact with biomolecules of interest;
• (c) wherein the presence of a target bound to the oligonucleotide in the fluorous-modified oligonucleotide composition or biomolecule interaction with the oligonucleotide in the fluorous-modified oligonucleotide composition is detectable by mass spectrometry through individual laser ionization of the oligonucleotide fluorous-modified composition immobilized on the perfluorocarbon covalently-modified electrically conductive surface.