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US 10,443,062 B2Granted
Genes that improve tolerance to lignocellulosic toxins when overexpressed in yeast
AssigneeWisconsin Alumni Research Foundation
InventorsMaria Sardi, Audrey P. Gasch
Life of this patent
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Abstract
The present invention provides isolated gene sequences useful in increasing lignocellulosic toxin tolerance in yeast. Such engineered yeast are useful in methods of biofuel production, particularly ethanol production. Methods of bioengineering recombinant yeast with increased lignocellulosic toxin tolerance are also provided.
Claims10
A recombinant vector comprising:
- (a) the nucleotide sequence of SEQ ID NO:1, SEQ ID NO:2, or SEQ ID NO:3;
- and (b) a promoter operably-linked to the nucleotide sequence of (a);
- wherein overexpression in yeast of said nucleotide sequence provides increased tolerance to lignocellulosic toxins relative to a control yeast lacking overexpression of the nucleotide sequence.
A recombinant Saccharomyces cerevisiae strain, comprising:
- an isolated nucleotide sequence encoding and overexpressing an adenylylsulfate kinase (MET14), protein folding protein folding co-chaperone (MDJ1), or C3 sterol dehydrogenase (ERG26);
- wherein the isolated nucleotide sequence is contained in an extrachromosomal vector maintained at a high copy number in the strain, and said strain exhibits increased tolerance to lignocellulosic toxins relative to a control strain lacking the isolated nucleotide sequence.
Dependencies are inferred from the claim text.