# US 10,441,951 B2 · Microfluidic chip with sealed on-board reagent

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- Number: US 10,441,951 B2
- Document ID: US10441951B2
- Status: Granted
- Kind: B2
- Application number: 14/775,338
- Filed: 2014-03-13
- Published: 2016-02-11
- Granted: 2019-10-15
- Earliest claimed: 2013-03-14
- Assignees: Siemens Healthcare Diagnostics Inc.
- Applicants: Siemens Healthcare Diagnostics Inc.
- Inventors: Dafeng Chen; James E. Rasmussen; Manish Deshpande
- Web page: https://patentgrep.com/patent/US10441951B2

## CPC

- B01L 3/5027: {by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip}
- B01L 3/502715: {characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces}
- B01L 3/523: {with means for closing or opening}
- F16K 99/003: {Valves for single use only}
- B01L 3/502738: {characterised by integrated valves (throttle valves in microfluidic sample containers B01L3/502746)}
- B01L 2200/10: Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
- B01L 2200/16: Reagents, handling or storing thereof
- B01L 2300/0672: Integrated piercing tool
- B01L 2300/0816: Cards, e.g. flat sample carriers usually with flow in two horizontal directions
- B01L 2300/0867: Multiple inlets and one sample wells, e.g. mixing, dilution
- B01L 2300/123: Flexible; Elastomeric
- B01L 2400/0481: squeezing of channels or chambers
- B01L 2400/0683: mechanically breaking a wall or membrane within a channel or chamber
- F16K 2099/008: {Multi-layer fabrications}
- F16K 2099/0084: {Chemistry or biology, e.g. "lab-on-a-chip" technology}
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- F16K: VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING {(devices for emptying and evacuating the excess liquid in valves or conduits F16L55/07)}

## Abstract

A microfluidic product pouch assembly may be used in a microfluidic chip. The microfluidic product pouch may include a pouch surrounding an inner chamber and having a rupturing portion and an inner membrane positioned within the inner chamber. The inner membrane may separate the inner chamber into a first cavity and a second cavity. A reagent may be positioned within the first cavity and/or the second cavity. The microfluidic product pouch assembly may also include a rupturing structure. The rupturing structure may be configured to selectively break the rupturing portion of the microfluidic product pouch.

## Claims

### Claim 1

A microfluidic chip, comprising:
a housing;
a sample port supported by the housing for introducing fluids into the housing;
one or more channels providing fluid communication between the sample port and a detection chamber; and,
one or more product pouch assemblies positioned adjacent to the one or more channels and housing one or more reagent;
wherein the product pouch assemblies have a pouch forming an inner chamber, the inner chamber housing the one or more reagents, at least a portion of the pouch having a rupturing portion, the rupturing portion positioned in fluid communication with the one or more reagent and the one or more channels,
wherein the inner chamber includes two or more cavities, the cavities separated by an inner membrane,
wherein one of the two or more cavities is a first cavity housing the one or more reagent, the one or more reagent in the first cavity having a dry formulation, the first cavity in fluid communication with the rupturing portion, the first cavity having a first pressure,
wherein another one of the two or more cavities is a second cavity housing a diluent which, when said inner membrane is pierced, penetrated and/or broken at approximately the same time or at a time shortly thereafter, as breaking the rupturing portion of the pouch, hydrates the one or more reagent in the first cavity having the dry formulation.

### Claim 2 (depends on 1)

The microfluidic chip of claim 1, wherein another one of the two or more cavities is a second cavity housing pressurized gas.

### Claim 3 (depends on 1)

The microfluidic chip of claim 1, further comprising, a pouch rupturing structure configured to open the one or more product pouch assemblies to release the one or more reagents into the one or more channels.

### Claim 4 (depends on 1)

The microfluidic chip of claim 1, wherein at least one of the channels has a first channel segment and a second channel segment separated by at least one of the one or more pouch assemblies such that rupturing at least two rupturing portions of the at least one pouch assembly connects the first channel segment to the second channel segment.

### Claim 5

A microfluidic chip, comprising:
a housing;
a sample port supported by the housing for introducing fluids into the housing;
one or more channels providing fluid communication between the sample port and a detection chamber; and,
one or more product pouch assemblies positioned adjacent to the one or more channels and housing one or more reagents;
wherein the product pouch assemblies have a pouch forming an inner chamber, at least a portion of the pouch having a rupturing portion, the rupturing portion positioned in fluid communication with the one or more reagents and the one or more channels,
wherein the inner chamber includes two or more cavities, the cavities separated by an inner membrane,
wherein one of the two or more cavities is a first cavity housing the one or more reagent, the first cavity in fluid communication with the rupturing portion,
wherein another one of the two or more cavities is a second cavity housing a diluent which, when said inner membrane is piereced, penetrated and/or broken at approximately the same time or at a time shortly thereafter, as breaking the rupturing portion of the pouch, hydrates the one or more reagent in the first cavity having the dry formulation,
wherein at least one of the channels has a first channel segment and a second channel segment separated by at least one of the one or more pouch assemblies such that rupturing at least two rupturing portions of the at least one pouch assembly connects the first channel segment to the second channel segment.