# US 10,441,950 B2 · Method for transferring a target between locations

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- Number: US 10,441,950 B2
- Document ID: US10441950B2
- Status: Granted
- Kind: B2
- Application number: 16/037,153
- Filed: 2018-07-17
- Published: 2018-11-08
- Granted: 2019-10-15
- Earliest claimed: 2014-01-14
- Assignees: Wisconsin Alumni Research Foundation
- Applicants: Wisconsin Alumni Research Foundation
- Inventors: David J. Beebe; Ben P. Casavant; David J. Guckenberger; Scott M. Berry
- Web page: https://patentgrep.com/patent/US10441950B2

## CPC

- B01L 3/0268: {using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries}
- B01L 3/50825: {Closing or opening means, corks, bungs}
- B01L 3/5088: {confining liquids at a location by surface tension, e.g. virtual wells on plates, wires (B01L3/50857 takes precedence)}
- B01L 3/563: {Joints or fittings; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors}
- B01L 2200/025: Align devices or objects to ensure defined positions relative to each other
- B01L 2200/0657: Pipetting powder
- B01L 2200/0668: Trapping microscopic beads
- B01L 2300/042: Caps; Plugs
- B01L 2400/021: non contact spotting by inertia, i.e. abrupt deceleration of the nozzle or pin
- B01L 2400/043: magnetic forces
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

## Abstract

A device and method are proved for transferring a target from a first location to a second location. The target is bound to solid phase substrate to form a target bound solid phase substrate. The device includes transfer surface for receiving the target bound solid phase substrate thereon for transfer. The transfer surface movable between a first position wherein the transfer surface is aligned with the first location and spaced therefrom by a distance and a second position wherein the transfer surface is aligned with the second location. An alignment structure aligns the transfer surface with respect to the second location, with the transfer surface in the second position. A force is movable between an attraction position wherein the target bound solid phase substrate are drawn toward the transfer surface and a discharge position wherein the target bound solid phase substrate are free of the force.

## Claims

### Claim 1

A method for transferring a target from a first fluid at a first location to a second location, the target being bound to a solid phase substrate to form a target bound solid phase substrate, comprising:
forming a non-concave meniscus with the first fluid;
providing a transfer device with a lower transfer surface and an upper surface;
positioning a transfer region of the lower transfer surface of the transfer device in axial alignment with and in spaced relation to the non-concave meniscus so as to provide a non-liquid air gap therebetween;
generating a force adjacent to the upper surface of the transfer device that is sufficient to attract the target bound solid phase substrate toward the transfer region;
drawing the target bound solid phase substrate from the first fluid, through the non-liquid air gap, and to the transfer region of the transfer device with the force;
maintaining the target bound solid phase substrate adjacent to the transfer region of the transfer device with the force;
aligning the transfer region with the second location, spaced from the first location;
reducing the force on the target bound solid phase substrate such that the force is insufficient to maintain the target bound solid phase substrate adjacent to the transfer region of the transfer device so as to release the target bound solid phase substrate from the transfer region of the transfer device and allow the target bound solid phase substrate to pass to the second location.

### Claim 2 (depends on 1)

The method of claim 1 wherein the step of forming the non-concave meniscus with the first fluid includes the additional steps of:
depositing the first fluid in a sample container; and
inserting an insert into the sample container, the insert causing the first fluid in the sample container to have the non-concave meniscus.

### Claim 3 (depends on 1)

The method of claim 1 wherein the step of forming the non-concave meniscus with the first fluid includes the additional step of depositing the first fluid in a sample container having a hydrophobic inner surface, the hydrophobic inner surface causing the first fluid in the sample container to have the non-concave meniscus.

### Claim 4 (depends on 1)

The method of claim 1 comprising the additional steps of:
positioning an intermediate fluid between the first fluid and the transfer region; and
passing the target bound solid phase substrate through the intermediate fluid as the target bound solid phase substrate are drawn from the first fluid to the transfer region.