# US 10,441,642 B2 · Immunogenic compositions and vaccines for preventing or treating filarial disease

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- Number: US 10,441,642 B2
- Document ID: US10441642B2
- Status: Granted
- Kind: B2
- Application number: 15/558,336
- Filed: 2016-03-17
- Published: 2018-03-08
- Granted: 2019-10-15
- Earliest claimed: 2015-03-18
- Assignees: The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc.; The United States Government as represented by the Secretary of the Department of Health and Human Services
- Applicants: The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc.; The United States Government, as Represented by the Secretary of the Department of Health and Human
- Inventors: Edward E. Mitre; Christopher Morris; Sasisekhar Bennuru; Thomas Nutman
- Web page: https://patentgrep.com/patent/US10441642B2

## CPC

- A61K 39/0003: {Invertebrate antigens}
- A61K 38/1767: {from invertebrates}
- A61K 39/39: characterised by the immunostimulating additives, e.g. chemical adjuvants
- A61P 33/10: Anthelmintics
- C07K 14/4354: {from nematodes}
- A61K 2039/55566: {Emulsions, e.g. Freund's adjuvant, MF59}
- Y02A 50/30: Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

The present disclosure is directed to an immunogenic composition including: at least one or at least two isolated polypeptides or immunogenic fragments thereof, and optionally a pharmaceutically acceptable carrier, wherein each polypeptide is expressed on a luminal surface of an intestine of a filarial worm, wherein each polypeptide is expressed at a level at least two-fold higher in the intestine in comparison to the level of expression of each polypeptide in a reproductive tract or a body wall of the filarial worm, wherein each isolated polypeptide has at least one transmembrane domain, and wherein each polypeptide is a non-mitochondrial polypeptide. Also provided herein is a method for preventing or treating a filarial disease.

## Claims

### Claim 1

An immunogenic composition comprising:
at least two isolated polypeptides or immunogenic fragments thereof and an adjuvant, wherein the at least two isolated polypeptides comprise a first polypeptide comprising the amino acid sequence of SEP ID NO: 1 and a second polypeptide comprising the amino acid sequence of SEP ID NO: 9 and the immunogenic fragments thereof comprise at least 30 contiguous amino acid sequences from the amino acid sequence of SEQ ID NO:1 and at least 30 contiguous amino add sequences from the amino acid sequence of SEQ ID NO: 9.

### Claim 2 (depends on 1)

The immunogenic composition as in claim 1, further comprising one or more polypeptides having an amino acid sequence selected from the group consisting of SEQ ID NOS: 2-7, 11, 14, 15, 18, 19, 22, 23 and 26.

### Claim 3 (depends on 1)

The immunogenic composition as in claim 1, wherein the immunogenic composition consists essentially of five isolated polypeptides and the adjuvant.

### Claim 4 (depends on 1)

The immunogenic composition as in claim 1, wherein the immunogenic composition is a vaccine.

### Claim 5 (depends on 1)

The immunogenic composition as in claim 1, wherein the adjuvant is Freund's complete adjuvant.

### Claim 6 (depends on 1)

A method for treating a filarial disease comprising administering an effective amount of a vaccine comprising the immunogenic composition of claim 1.

### Claim 7 (depends on 6)

The method of claim 6, wherein the filarial disease is selected from the group consisting of lymphatic filariasis, river blindness, loiasis and heartworm.

### Claim 8 (depends on 6)

The method of claim 6, wherein the subject is a human.

### Claim 9 (depends on 6)

The method of claim 6, wherein the filarial disease is heartworm.

### Claim 10 (depends on 6)

The method of claim 6, wherein the subject is a dog.

### Claim 11 (depends on 6)

The method of claim 6, wherein the vaccine is subcutaneously, intradermally, orally, or nasally administered.