# US 10,441,632 B2 · Anti-wrinkle and anti-aging cosmetic composition comprising green fluorescent protein-human epidermal growth factor fusion protein as effective component

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- Number: US 10,441,632 B2
- Document ID: US10441632B2
- Status: Granted
- Kind: B2
- Application number: 16/071,009
- Filed: 2017-01-25
- Published: 2019-01-24
- Granted: 2019-10-15
- Earliest claimed: 2016-01-29
- Assignees: Nexgen Biotechnologies, Inc.; Sun Kyo Lee
- Applicants: Nexgen Biotechnologies, Inc.; Sun Kyo Lee
- Inventors: Sun Kyo Lee; Seong Ran Lee; Han Bong Ryu; Tae Hyun Kim; Tae Won Choi; Hyeong Il Kwon
- Web page: https://patentgrep.com/patent/US10441632B2

## CPC

- A61K 38/1808: {Epidermal growth factor [EGF] urogastrone}
- A61K 8/02: characterised by special physical form
- A61K 8/64: Proteins; Peptides; Derivatives or degradation products thereof
- A61K 8/98: of animal origin
- A61K 8/987: {of species other than mammals or birds}
- A61K 38/1767: {from invertebrates}
- A61Q 19/08: Anti-ageing preparations
- C07K 14/43595: {from coelenteratae, e.g. medusae}
- C07K 14/485: Epidermal growth factor [EGF], i.e. urogastrone
- C07K 14/43504: {from invertebrates}
- C07K 2319/00: Fusion polypeptide
- C07K 2319/60: containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

A method for treating wrinkle or aging on a skin of a subject includes applying to the skin a composition that includes a green fluorescent protein-human epidermal growth factor fusion protein as an effective component. Since the fusion protein has an excellent anti-oxidation activity and a high skin cell proliferation effect, it can be advantageously used as a raw material of functional cosmetics having excellent skin regeneration effect like improvement of skin wrinkles and skin whitening.

## Claims

### Claim 1

A method for treating wrinkle or aging on a skin of a subject, the method comprising applying to the skin of the subject a composition comprising a green fluorescent protein-human epidermal growth factor fusion protein, wherein the green fluorescent protein-human epidermal growth factor fusion protein consists of the amino acid sequence of SEQ ID NO: 2.

### Claim 2 (depends on 1)

The method of claim 1, wherein the green fluorescent protein-human epidermal growth factor fusion protein is included at 0.000002 to 0.002% by weight relative to the total weight of the composition.

### Claim 3 (depends on 1)

The method of claim 1, wherein the composition has any one formulation selected from a group consisting of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage crème, a nutrition crème, an eye créme, a moisturizing créme, a hand créme, an essence, a nutrition essence, a pack, a cleansing foam, a cleansing water, a cleansing lotion, a cleansing créme, a body lotion, a body cleanser, a soap, and a powder.