# US 10,441,615 B2 · Method for preparing fresh chlorella drink and use of the fresh chlorella drink

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- Number: US 10,441,615 B2
- Document ID: US10441615B2
- Status: Granted
- Kind: B2
- Application number: 15/598,306
- Filed: 2017-05-17
- Published: 2018-11-22
- Granted: 2019-10-15
- Earliest claimed: 2017-05-17
- Applicants: Ting-Jung Lin; Mei-Hua Huang
- Inventors: Ting-Jung Lin; Mei-Hua Huang
- Web page: https://patentgrep.com/patent/US10441615B2

## CPC

- A61K 36/05: Chlorophycota or chlorophyta (green algae), e.g. Chlorella
- A23L 2/52: Adding ingredients (adding preservatives A23B70/10)
- A23L 17/60: Edible seaweed
- A23L 33/105: Plant extracts, their artificial duplicates or their derivatives
- A23L 33/30: {Dietetic or nutritional methods, e.g. for losing weight (A23L33/10 takes precedence)}
- A61K 9/0095: {Drinks; Beverages; Syrups; Compositions for reconstitution thereof, e.g. powders or tablets to be dispersed in a glass of water; Veterinary drenches (A61K9/0007 takes precedence; eatable gels or foams A61K9/0056; oral mucosa adhesive forms A61K9/006)}
- C12N 1/12: Unicellular algae; Culture media therefor (as new plants A01H13/00)
- A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION

## Abstract

Provided is a method for preparing a fresh Chlorella drink by sterile culturing and freezing and then rapidly raising the temperature.

## Claims

### Claim 1

A method for preparing a fresh Chlorella drink, comprising the steps of:
(a) sterilely culturing and stirring Chlorella for not longer than 3 days, until the concentration of the Chlorella is over 0.05% by weight,
(b) harvesting the culture of Chlorella within 6 hours;
(c) cooling the harvested culture obtained in step (b) to lower than 1° C. but not to a frozen state, then
(d) centrifuging the cooled culture obtained in step (c), and
(e) adding sterile water to obtain a Chlorella suspension, wherein the concentration of the Chlorella is from 2.5 mg/ml to 12.5 mg/ml; and
(f) freezing the Chlorella suspension obtained in step (e) to be completely frozen for 12 hours to 24 hours and then
(g) increasing the temperature to 40° C. to 50° C. within 10 minutes to obtain the fresh Chlorella drink.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the stirring in step (a) is from 3 m/min to 20 m/min.

### Claim 3 (depends on 1)

The method according to claim 1, wherein, in step (g), the temperature is raised by microwaves or infrared rays.

### Claim 4 (depends on 1)

The method according to claim 1, wherein, in step (f), the frozen temperature ranges from −1° C. to −21° C.

### Claim 5 (depends on 1)

The method according to claim 1, wherein, in step (g), the period of raising the temperature is from 3 minutes to 10 minutes.