# US 10,441,594 B2 · Methods of making quinoa leachates and uses thereof

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- Number: US 10,441,594 B2
- Document ID: US10441594B2
- Status: Granted
- Kind: B2
- Application number: 14/562,238
- Filed: 2014-12-05
- Published: 2015-06-11
- Granted: 2019-10-15
- Earliest claimed: 2013-12-06
- Assignees: Rutgers, The State University of New Jersey
- Applicants: Rutgers, the State University of New Jersey
- Inventors: Ilya Raskin; Brittany Graf
- Web page: https://patentgrep.com/patent/US10441594B2

## CPC

- A61K 31/575: substituted in position 17 beta by a chain of three or more carbon atoms, e.g. cholane, cholestane, ergosterol, sitosterol
- A61K 36/21: Amaranthaceae (Amaranth family), e.g. pigweed, rockwort or globe amaranth
- A61K 2236/00: Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

The present application is directed to materials and method for producing leachates from a plant of the Amaranthaceae family.

## Claims

### Claim 1

A method of producing a leachate comprising 20-hydroxyecdysone from an intact quinoa seed comprising:
contacting the intact quinoa seed with a leaching fluid to produce a leaching mixture comprising the 20-hydroxyecdysone, wherein the leaching fluid is selected from the group consisting of water, n-butanol, isopropanol, n-propanol, aqueous ethanol having a concentration less than 95% ethanol, methanol, ethyl acetate, nitromethane and formic acid, and wherein the contacting step is performed at a temperature of less than 80° C.; and
separating the quinoa seed from the leaching mixture, thereby producing a leachate comprising 20-hydroxyecdysone from the quinoa seed, wherein the leachate comprises from about 100 μg to about 400 μg 20-hydroxyecdysone per gram of seed.

### Claim 2 (depends on 1)

The method of claim 1, wherein the quinoa seed is a Chenopodium quinoa plant seed.

### Claim 3 (depends on 1)

The method of claim 1, wherein the leaching fluid comprises 60-80% ethanol.

### Claim 4 (depends on 3)

The method of claim 3, wherein the leaching fluid comprises 70% ethanol.

### Claim 5 (depends on 1)

The method of claim 1, wherein the contacting step is performed at room temperature.

### Claim 6 (depends on 1)

The method of claim 1, wherein the separating step is performed using a method selected from the group consisting of filtration, sedimentation, centrifugation, evaporation, reduced-pressure distillation, precipitation, lyophilization and adsorption.

### Claim 7 (depends on 1)

The method of claim 1, further comprising drying the leachate.

### Claim 8 (depends on 7)

The method of claim 7, wherein the drying is performed by using a method selected from the group consisting of air drying, spray drying, speed vacuum, rotoevaporation, and lyophilization.