# US 10,440,975 B2 · Feed additive composition for reducing methane gas produced by ruminant animals > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,440,975 B2 - Document ID: US10440975B2 - Status: Granted - Kind: B2 - Application number: 15/315,593 - Filed: 2015-06-02 - Published: 2017-07-13 - Granted: 2019-10-15 - Earliest claimed: 2014-06-02 - Assignees: Cj Cheiljedang Corporation - Applicants: Cj Cheiljedang Corporation - Inventors: Min Ah Park; Seung Heon Lee; Sung Hun Kim; In Bae Kim; Si Yong Yang; Seok Woo Chee - Web page: https://patentgrep.com/patent/US10440975B2 ## CPC - A23K 50/10: for ruminants - A23K 20/10: Organic substances - A23K 20/111: Aromatic compounds - A23K 20/121: containing oxygen or sulfur as hetero atom - A23K 20/137: containing two hetero atoms, of which at least one is nitrogen - A23K 20/142: Amino acids; Derivatives thereof - A23K 10/30: from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms (obtained by microbiological or biochemical processes, e.g. using yeasts or enzymes, A23K10/10) - Y02P 60/22: Methane [CH4], e.g. from rice paddies - A23K: FODDER - Y02P: CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS ## Abstract The present disclosure relates to a feed additive composition for reducing methane production generated in the ruminant stomach of ruminant animals. Specifically, the present disclosure relates to a feed additive composition for reducing methane production comprising at least one selected from the group consisting of alliin and berberine, more specifically to a feed additive composition for reducing methane production comprising at least one selected from the group consisting of diallyl disulfide (DADS), nitrate, and eucalyptus oil. The feed additive composition according to the present disclosure may be added to the feed without additional treatment, thereby reducing the amount of methane production in the ruminant stomach without negative effects on livestock productivity. Thus, the feed additive composition can be effectively used in the ruminant animal industry. ## Claims ### Claim 1 A feed additive composition for reducing methane production comprising berberine, nitrate, and eucalyptus oil, wherein the composition is added to a feed to comprise berberine in an amount of 0.01 wt % to 5 wt % relative to the weight of dried feed. ### Claim 2 (depends on 1) The composition according to claim 1, wherein the composition is added to a feed to comprise nitrate in an amount of 0.01 wt % to 0.5 wt %, and eucalyptus oil in an amount of 0.01 wt % to 5 wt % relative to the weight of dried feed. ### Claim 3 (depends on 1) The composition according to claim 1, wherein the composition further comprises at least one selected from the group consisting of diallyl disulfide (DADS) and alliin. ### Claim 4 (depends on 3) The composition according to claim 3, wherein the composition is added to a feed to comprise diallyl disulfide in an amount of 0.005 wt % to 4 wt % or alliin in an amount of 0.005 wt % to 5 wt % relative to the weight of dried feed. ### Claim 5 (depends on 4) The composition according to claim 4, wherein the composition is added to a feed to comprise diallyl disulfide in an amount of 0.005 wt % to 4 wt %, nitrate in an amount of 0.01 wt % to 0.5 wt %, berberine in an amount of 0.01 wt % to 5 wt %, and eucalyptus oil in an amount of 0.01 wt % to 5 wt % relative to the weight of dried feed. ### Claim 6 (depends on 4) The composition according to claim 4, wherein the composition is added to a feed to comprise alliin in an amount of 0.005 wt % to 5 wt %, nitrate in an amount of 0.01 wt % to 0.5 wt %, berberine in an amount of 0.01 wt % to 5 wt %, and eucalyptus oil in an amount of 0.01 wt % to 5 wt % relative to the weight of dried feed. ### Claim 7 A reducing method for of methane production comprising administering a composition comprising berberine to a subject. ### Claim 8 (depends on 7) The reducing method according to claim 7, wherein the composition is a feed additive composition and further comprises alliin. ### Claim 9 (depends on 8) The reducing method according to claim 8, wherein the composition is a feed additive composition and comprises berberine in an amount of 0.01 wt % to 5 wt % and alliin in an amount of 0.005 wt % to 5 wt % relative to the weight of dried feed. ### Claim 10 (depends on 7) The reducing method according to claim 7, wherein the composition further comprises at least one selected from the group consisting of diallyl disulfide (DADS), alliin, nitrate, and eucalyptus oil. ### Claim 11 (depends on 10) The reducing method according to claim 10, wherein the composition is a feed additive composition and comprise diallyl disulfide in an amount of 0.005 wt % to 4 wt %, alliin in an amount of 0.005 wt % to 5 wt %, nitrate in an amount of 0.01 wt % to 0.5 wt %, berberine in an amount of 0.01 wt % to 5 wt %, or eucalyptus oil in an amount of 0.01 wt % to 5 wt % relative to the weight of dried feed. ### Claim 12 (depends on 10) The reducing method according to claim 10, wherein the composition comprises diallyl disulfide, nitrate, berberine, and eucalyptus oil. ### Claim 13 (depends on 12) The reducing method according to claim 12, wherein the composition is a feed additive composition and comprises diallyl disulfide in an amount of 0.005 wt % to 4 wt %, nitrate in an amount of 0.01 wt % to 0.5 wt %, berberine in an amount of 0.01 wt % to 5 wt %, and eucalyptus oil in an amount of 0.01 wt % to 5 wt % relative to the weight of dried feed. ### Claim 14 (depends on 10) The reducing method according to claim 10, wherein the composition comprises alliin, nitrate, berberine, and eucalyptus oil. ### Claim 15 A feed additive composition for reducing methane production comprising berberine, nitrate, and eucalyptus oil, wherein the composition is added to a feed to comprise berberine in an amount of 0.05 wt % to 5 wt % relative to the weight of dried feed. ### Claim 16 (depends on 13) The reducing method according to claim 13, wherein the composition is added to a feed to comprise alliin in an amount of 0.005 wt % to 5 wt %, nitrate in an amount of 0.01 wt % to 0.5 wt %, berberine in an amount of 0.01 wt % to 5 wt %, and eucalyptus oil in an amount of 0.01 wt % to 5 wt % relative to the weight of dried feed.