# US 10,440,974 B2 · Methionine-rich composition for feeding animals

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- Number: US 10,440,974 B2
- Document ID: US10440974B2
- Status: Granted
- Kind: B2
- Application number: 15/399,764
- Filed: 2017-01-06
- Published: 2017-04-27
- Granted: 2019-10-15
- Earliest claimed: 2009-10-14
- Assignees: Evonik Degussa GmbH
- Applicants: Evonik Degussa GmbH
- Inventors: Patrick Fuertes
- Web page: https://patentgrep.com/patent/US10440974B2

## CPC

- A23K 20/142: Amino acids; Derivatives thereof
- A23L 5/20: Removal of unwanted matter, e.g. deodorisation or detoxification
- C12P 13/06: Alanine; Leucine; Isoleucine; Serine; Homoserine
- C12P 13/12: Methionine; Cysteine; Cystine
- A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs
- A23K: FODDER
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION

## Abstract

The present invention relates to a novel liquid or crystalline methionine composition, obtained by means of biomass bioconversion by means of microorganisms that are suitable for producing methionine.

## Claims

### Claim 1

A feed additive for feeding animals comprising a methionine composition resulting from a fermentation process, said methionine composition comprising:
from 60 to 95% by weight of methionine;
from 0.05 to 2.5% by weight of N-acetylmethionine; and
from 0.05 to 3.5% by weight of isoleucine.

### Claim 2 (depends on 1)

The feed additive of claim 1, said methionine composition further comprising:
from 1.0 to 6.0% by weight of amino acids other than methionine, N-acetylmethionine and isoleucine;
from 0.1 to 2% by weight of total sugars; and
from 0.05 to 2.5% by weight of lipids.

### Claim 3 (depends on 1)

The feed additive of claim 1, wherein said additive is a liquid.

### Claim 4 (depends on 1)

The feed additive of claim 1, wherein said methionine composition comprises:
from 60 to 80% by weight of methionine;
from 0.5 to 2.0% by weight of N-acetylmethionine; and
from 0.20 to 1.5% by weight isoleucine.

### Claim 5 (depends on 2)

The feed additive of claim 2, said methionine composition further comprising:
from 2 to 6% by weight of amino acids other than methionine, N-acetylmethionine and isoleucine;
from 0.5 to 2% by weight of total sugars; and
from 0.05 to 2.5% by weight of lipids.

### Claim 6 (depends on 1)

The feed additive of claim 1, said methionine composition comprising:
from 70 to 90% by weight of methionine;
from 0.05 to 2.5% by weight of N-acetylmethionine; and
from 0.10 to 1.0% by weight of isoleucine.

### Claim 7 (depends on 2)

The feed additive of claim 2, said methionine composition further comprising:
from 2 to 6% by weight of amino acids other than methionine, N-acetylmethionine and isoleucine;
from 0.25 to 2% by weight of total sugars; and
from 0.05 to 2.5% by weight of lipids.

### Claim 8 (depends on 2)

The feed additive of claim 2, wherein said additive is a liquid.

### Claim 9 (depends on 1)

The feed additive of claim 1, said methionine composition comprising:
from 80 to 95% by weight of methionine;
from 0.05 to 0.5% by weight of N-acetylmethionine; and
from 0.05 to 5.0% by weight of isoleucine.

### Claim 10 (depends on 2)

The feed additive of claim 2, said methionine composition further comprising:
from 1.0 to 5.0% by weight of amino acids other than methionine, N-acetylmethionine and isoleucine;
from 0.1 to 1.5% by weight of total sugars, and less than 0.3% by weight of lipids.

### Claim 11 (depends on 1)

A process for preparing the feed additive of claim 1 comprising:
clarifying fermentation medium in which a microorganism that has been modified to produce methionine has been cultured and removing insoluble and soluble organic impurities from said fermentation medium to obtain a liquid crude composition; and
recovering the feed additive.

### Claim 12 (depends on 11)

The process for preparing a feed additive of claim 11, wherein the liquid crude composition further comprises:
from 2 to 6% by weight of amino acids other than methionine, N-acetylmethionine and isoleucine;
from 0.1 to 2% by weight of total sugars; and
from 0.05 to 2.5% by weight of lipids.

### Claim 13 (depends on 11)

The process for preparing a feed additive of claim 11, said process further comprising:
demineralizing the liquid crude solution in order to remove cations and anions from said fermentation medium to obtain a demineralized liquid crude composition comprising:
from 70 to 90% by weight of methionine;
from 0.05 to 2.5% by weight of N-acetylmethionine; and
from 0.10 to 1.0% by weight of isoleucine.

### Claim 14 (depends on 13)

The process for preparing a feed additive of claim 13, said demineralized liquid crude composition further comprising:
from 2 to 6% by weight of amino acids other than methionine, N-acetylmethionine and isoleucine;
from 0.25 to 2% by weight of total sugars; and
from 0.05 to 2.5% by weight of lipids.

### Claim 15 (depends on 11)

The process for preparing a feed additive of claim 11, wherein the feed additive is liquid and said process does not comprise a crystallization step.

### Claim 16 (depends on 11)

The process for preparing a feed additive of claim 11, further comprising:
crystallizing solid crude methionine from an optionally demineralized, liquid crude composition.

### Claim 17 (depends on 16)

The process for preparing a feed additive of claim 16, said method further comprising:
separating, optionally washing, and optionally milling methionine crystals in order to obtain a solid crude composition.

### Claim 18 (depends on 16)

The process for preparing a feed additive of claim 16, wherein said solid crude composition comprises:
from 80 to 95% by weight of methionine;
from 0.05 to 0.5% by weight of N-acetylmethionine; and
from 0.05 to 5.0% by weight of isoleucine.

### Claim 19 (depends on 18)

The process for preparing a feed additive of claim 18, wherein the solid crude methionine composition further comprises:
from 1.0 to 5.0% by weight of amino acids other than methionine, N-acetylmethionine and isoleucine;
from 0.1 to 1.5% by weight of total sugars; and
less than 0.3% by weight of lipids.