# US 10,440,947 B2 · Aqueous solution for preserving an organ and uses during hypothermic ischemia

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 10,440,947 B2
- Document ID: US10440947B2
- Status: Granted
- Kind: B2
- Application number: 15/036,432
- Filed: 2014-11-13
- Published: 2016-10-13
- Granted: 2019-10-15
- Earliest claimed: 2013-11-15
- Assignees: Inserm (Institut National De La Sante Et De La Recherche Medicale); Universite Claude Bernard Lyon I
- Applicants: Universite Claude Bernard Lyon I; Inserm (Institut National De La Sante Et De La Recherche Medicale)
- Inventors: Rene Ferrera
- Web page: https://patentgrep.com/patent/US10440947B2

## CPC

- A01N 1/126: Physiologically active agents, e.g. antioxidants or nutrients
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS

## Abstract

The present invention relates to an aqueous solution for organ preservation, in particular for the myocardium, the solution comprising: 100 mM to 130 mM of Na+; 12 mM to 15 mM of K+; 0.25 mM to 1.3 mM of Ca++; 0.5 μM to 1.5 μM of cyclosporin A; and 0.5 μM to 1.5 μM of taxol, and it also relates to the joint use of cyclosporin A and taxol in a solution for in vitro preservation of an organ, in particular the myocardium.

## Claims

### Claim 1

A solution for organ preservation, the solution comprising:
100 mM to 130 mM of Na+;
12 mM to 15 mM of K+;
0.25 mM to 1.3 mM of Ca++;
0.5 μM to 1.5 μM of cyclosporin A; and
0.5 μM to 1.5 μM of taxol;
wherein cyclosporin A and taxol act synergistically, and provide an anti-ischemic action.

### Claim 2 (depends on 1)

The solution according to claim 1, comprising:
110 to 130 mM of Na+;
12 to 14 mM of K+; and
0.7 to 1.3 mM of Ca++.

### Claim 3 (depends on 1)

The solution according to claim 1, further comprising magnesium Mg++.

### Claim 4 (depends on 1)

The solution according to claim 1, further comprising:
polyethylene glycol having a mean molecular weight of 7200 to 8800 daltons; and
polyethylene glycol having a mean molecular weight of 18,000 to 22,000 daltons.

### Claim 5 (depends on 1)

The solution according to claim 1, further comprising insulin and glucose.

### Claim 6 (depends on 1)

The solution according to claim 1, further comprising insulin and pyruvate.

### Claim 7 (depends on 1)

The solution according to claim 1, further comprising pyruvate, aspartate, and glutamate.

### Claim 8 (depends on 1)

The solution according to claim 1, further comprising procaine.

### Claim 9 (depends on 1)

The solution according to claim 1, further comprising adenosine.

### Claim 10 (depends on 1)

The solution according to claim 1, further comprising reduced glutathione.

### Claim 11 (depends on 1)

The solution according to claim 1, comprising:
110 to 130 mM of Na+;
12 to 14 mM of K+;
0.7 to 1.3 mM of Ca++;
0.5 to 1.5 μM of cyclosporin A;
0.5 to 1.5 μM of taxol;
3.5 to 4.5 mM of Mg++;
118 to 142 mM of Cl−;
1.5 to 2.5 g/L of polyethylene glycol having a mean molecular weight of 7200 to 8800 daltons;
2 to 4 g/L of polyethylene glycol having a mean molecular weight of 18,000 to 22,000 daltons;
240 to 260 IU/L of insulin;
19 to 21 mM of glucose;
2.4 to 2.6 mM of pyruvate;
1 to 3 mM of aspartate;
0.4 to 2.4 mM of glutamate;
0.5 to 1.5 mM of procaine; and
water.

### Claim 12 (depends on 1)

The solution according to claim 1, comprising 0.7 to 1.3 μM of taxol and 0.7 to 1.3 μM of cyclosporin A.

### Claim 13 (depends on 1)

The solution according to claim 1, comprising:
120 mM of Na+;
13 mM of K+;
1 mM of Ca++;
1 μM of cyclosporin A;
1 μM of taxol;
4 mM of Mg++;
130 mM of Cl−;
2 g/L of polyethylene glycol having a mean molecular weight of 7200 to 8800 daltons;
3 g/L of polyethylene glycol having a mean molecular weight of 18,000 to 22,000 daltons;
250 IU/L of insulin;
20 mM of glucose;
2.5 mM of pyruvate;
2 mM of aspartate;
1.4 mM of glutamate;
1 mM of procaine; and
water.

### Claim 14 (depends on 1)

The solution according to claim 1, wherein the solution has an osmolarity in a range of from 280 mOsm/L to 320 mOsm/L.

### Claim 15 (depends on 1)

The solution according to claim 1, wherein the solution is buffered to a pH of 7.3 to 7.4.

### Claim 16 (depends on 15)

The solution according to claim 15, wherein the solution is buffered with HEPES solution.

### Claim 17 (depends on 1)

The solution according to claim 1, further comprising a compound for increasing membrane fluidity.