# US 10,440,919 B2 · Variety corn line HFA4510

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- Number: US 10,440,919 B2
- Document ID: US10440919B2
- Status: Granted
- Kind: B2
- Application number: 15/800,143
- Filed: 2017-11-01
- Published: 2019-05-02
- Granted: 2019-10-15
- Earliest claimed: 2017-11-01
- Assignees: Syngenta Participations AG
- Applicants: Syngenta Participations AG
- Inventors: Scott N. Kelly
- Web page: https://patentgrep.com/patent/US10440919B2

## CPC

- A01H 6/4684: {Zea mays [maize]}
- A01H 5/10: Seeds
- C11B 1/00: Production of fats or fatty oils from raw materials
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C11B: PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES (drying-oils C09F)

## Abstract

The present invention provides an inbred corn line designated HFA4510, methods for producing a corn plant by crossing plants of the inbred line HFA4510 with plants of another corn plant. The invention further encompasses all parts of inbred corn line HFA4510, including culturable cells. Additionally provided herein are methods for introducing transgenes into inbred corn line HFA4510, and plants produced according to these methods.

## Claims

### Claim 1

A seed of maize variety HFA4510, wherein representative seed of said maize variety HFA4510 has been deposited under ATCC Accession Number PTA-124534.

### Claim 2

A plant of maize variety HFA4510, wherein representative seed of said maize variety HFA4510 has been deposited under ATCC Accession Number PTA-124534.

### Claim 3 (depends on 2)

A plant part of the plant of claim 2.

### Claim 4 (depends on 3)

The plant part of claim 3, wherein said part is a pollen grain, a silk, a protoplast, a cell, a tassel, an anther or an ovule.

### Claim 5 (depends on 2)

A maize seed produced on the plant of claim 2.

### Claim 6 (depends on 2)

A maize plant having all of the physiological and morphological characteristics of the plant according to claim 2 and further comprising an additional trait, wherein the additional trait is selected from the group consisting of water stress resistance, waxy starch, male sterility or restoration of male fertility, modified carbohydrate metabolism, modified protein metabolism, modified fatty acid metabolism, altered starch, thermotolerant amylase, herbicide resistance, insect resistance, nematode resistance, bacterial disease resistance, fungal disease resistance, and viral disease resistance.

### Claim 7 (depends on 6)

The plant of claim 6 wherein the additional trait is conferred by introducing a transgene.

### Claim 8 (depends on 2)

A converted plant of claim 2, representative seed of the maize variety HFA4510 having been deposited under ATCC Accession Number PTA-124534, wherein the converted plant of maize variety HFA4510 further comprises a locus conversion conferring a particular trait and otherwise comprises all of the phenotypic characteristics of maize variety HFA4510 listed in Table 1 when grown under the same environmental conditions, except for those changed by the introduction of the locus.

### Claim 9 (depends on 2)

A process for producing maize seed, said process comprising: crossing the maize plant of claim 2 with a different maize plant, and harvesting the seed.

### Claim 10 (depends on 9)

An F1 maize seed produced by the process of claim 9.

### Claim 11 (depends on 10)

An F1 maize plant produced by germinating the seed of claim 10.

### Claim 12 (depends on 10)

A method of producing a genetic marker profile, comprising: extracting nucleic acids from the seed of claim 10 or the plant germinated from said seed, and genotyping said nucleic acids at one or more genetic loci, thereby producing a genetic marker profile.

### Claim 13 (depends on 10)

A method of plant breeding, comprising: a) isolating nucleic acids from the seed of claim 10, b) identifying one or more polymorphisms from the isolated nucleic acids, and c) selecting a plant obtained from said seed having said one or more polymorphisms, wherein the plant is used in a plant breeding method.

### Claim 14 (depends on 11)

A method of plant breeding, comprising: a) isolating nucleic acids from the plant of claim 11, b) identifying one or more polymorphisms from the isolated nucleic acids, and c) selecting a plant having said one or more polymorphisms, wherein the plant is used in a plant breeding method.

### Claim 15

A process of introducing an additional trait into maize plant HFA4510, comprising: (a) crossing HFA4510 plants grown from HFA4510 seeds, representative seeds deposited under ATCC Accession Number PTA-124534, with plants of another maize variety that comprise an additional trait to produce hybrid progeny plants, (b) selecting hybrid progeny plants that have the additional trait to produce selected hybrid progeny plants; (c) crossing the selected progeny plants with the HFA4510 plants to produce backcross progeny plants; (d) selecting for backcross progeny plants that have the additional trait and physical and morphological characteristics of maize variety HFA4510 to produce selected backcross progeny plants; and (e) repeating steps (c) and (d) at least three or more times to produce backcross progeny plants that comprise the additional trait and all of the physiological and morphological characteristics of maize inbred plant HFA4510 when grown in the same environmental conditions.

### Claim 16 (depends on 15)

A plant produced by the process of claim 15.

### Claim 17 (depends on 11)

A method of producing a maize plant derived from the inbred plant HFA4510, the method comprising the steps of (a) growing the plant of claim 11; (b) crossing said plant with itself or a different plant to produce a seed of a progeny plant; (c) repeating step (b) at least one or more times; and (d) growing said progeny plant from said seed and crossing the progeny plant with itself or a different plant to produce a maize plant derived from the inbred plant HFA4510.

### Claim 18 (depends on 11)

A method for developing a second maize variety in a maize plant breeding program, comprising: applying plant breeding techniques wherein said techniques comprise recurrent selection, backcrossing, pedigree breeding, marker enhanced selection, haploid/double haploid production, or transformation to the maize plant of claim 11, wherein application of said techniques results in development of a second maize variety.

### Claim 19 (depends on 10)

A method of producing a commodity plant product, comprising: growing the plant from the seed of claim 10, and producing said commodity plant product comprising protein concentrate, protein isolate, starch, meal, flour or oil therefrom.

### Claim 20 (depends on 11)

A method of producing a maize plant with doubled haploid chromosomes from the maize variety HFA4510, the method comprising: (a) crossing the plant of claim 11 with an inducer maize plant to produce a progeny with haploid chromosomes; and (b) doubling the haploid chromosomes in the progeny to produce a maize plant with doubled haploid chromosomes.