# US 10,440,914 B2 · Bremia lactucae resistant plants

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 10,440,914 B2
- Document ID: US10440914B2
- Status: Granted
- Kind: B2
- Application number: 15/262,071
- Filed: 2016-09-12
- Published: 2016-12-29
- Granted: 2019-10-15
- Earliest claimed: 2014-03-14
- Assignees: Rijk Zwaan Zaadteelt En Zaadhandel B.V.; Nunhems B.V.; Vilmorin & Cie
- Applicants: Rijk Zwaan Zaadteelt En Zaadhandel B.V.; Nunhems B.V.; Vilmorin & Cie
- Inventors: Marie Jeanette Willemijn Jeuken; Erik Den Boer; Richard Gerardus Franciscus Visser; Rients Engelhard Niks
- Web page: https://patentgrep.com/patent/US10440914B2

## CPC

- A01H 6/1472: {Lactuca sativa [lettuce]}
- A01H 1/021: {Methods of breeding using interspecific crosses, i.e. interspecies crosses}
- A01H 1/045: {using molecular markers}
- A01H 1/1255: {for fungal resistance}
- A01H 5/12: Leaves
- C12Q 1/6895: for plants, fungi or algae
- C12Q 2600/13: Plant traits
- C12Q 2600/156: Polymorphic or mutational markers
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The invention relates to a lettuce plant (Lactuca sativa L.) which may comprise a resistance allele which confers a broad spectrum resistance to Bremia lactucae, wherein the resistance allele is located on linkage group 9, and which resistance allele is as found in the genome of plants grown from seeds of which a representative sample was deposited under accession number NCIMB 42141. The invention further provides seeds, progeny, propagation material and food products from the plant.

## Claims

### Claim 1

A Lactuca sativa L. plant comprising an introgression fragment from L. saligna which confers a broad spectrum resistance to Bremia lactucae, wherein the introgression fragment comprises marker CLS_S3_3349 (SEQ ID NO:1) and marker CLS_S3_4656 (SEQ ID NO:3), wherein the introgression fragment is the fragment as found in seeds of which a representative sample was deposited under accession number NCIMB 42141.

### Claim 2 (depends on 1)

A seed capable of growing into the plant of claim 1.

### Claim 3 (depends on 1)

A seed produced on the plant of claim 1, wherein the seed comprises the introgression fragment.

### Claim 4 (depends on 1)

A progeny of the plant of claim 1, wherein the progeny plant comprises the introgression fragment.

### Claim 5 (depends on 1)

A propagation material derived from the plant of claim 1, wherein the propagation material comprises the introgression fragment.

### Claim 6 (depends on 5)

The propagation material of claim 5, wherein the propagation material comprises a microspore, pollen, ovary, ovule, embryo, embryo sac, egg cell, cutting, root, root tip, hypocotyl, cotyledon, stem, leaf, flower, anther, seed, meristematic cell, protoplast or cell.

### Claim 7 (depends on 5)

A tissue culture of the propagation material of claim 5.

### Claim 8 (depends on 1)

A part of the plant of claim 1, wherein the part is a harvested lettuce head or leaf, and wherein the part is optionally in processed form.

### Claim 9 (depends on 8)

A food product comprising the part of the plant of claim 8.

### Claim 10 (depends on 1)

A method of developing Lactuca sativa plants having a broad spectrum resistance to Bremia lactucae, said method comprising isolating germ plasm from the plant of claim 1 for a breeding program.

### Claim 11 (depends on 2)

A method for seed production, said method comprising growing a lettuce plant from the seed of claim 2, allowing the plant to produce seeds, and harvesting the seeds.